Company: Mark Andy Inc.
Job Description Electrical Assembler Come for a job and stay for a careeromPerks and Benefits at Mark Andy Inc. Competitive wages Tuition Reimbursement Generous paid time off Medical, dental and vision benefits 401k and cars Overtime paid after 40 hours a week Day shift schedule 6:00am - 2:30pm Second shift schedule 2:00pm - 10:30pm (shift differential for second shift schedule) Position Summary Perform duties required to assemble all electrical components into the main power packs with respect to each product line. Assemble main electrical power pack per design and engineering specifications using schematics Assemble electrical and pneumatic components, into sub-assemblies across multiple models in each respective product lines. Assemble various components into electrical and pneumatic sub-assemblies per engineering drawings, schematics and diagrams Responsible for completeness of sub-assemblies per the ATO sales order. Responsible for all sub assembly documentation. Insge Inspect completed sub-assemblies, visually and manually. Supports the assembly group in determining what is required to meet the sales order. Support engineering department in redesigning and building prototype sub-assemblies for presses Responsible for preparing the following forms when needed: material request, material return to stock, IRAD form, etc. Qualifications/ Skills Required Basic electrical skills Troubleshooting skills Work with little or no supervision High attention to detail Team-oriented Problem solving skills Personal computer s interpret blueprints Capable of performing assembly work with minimal assistance. Maintain a safe and organized work environment. of the position. Education/Experience Required High school degree or equivalent Experience: 6 months electrical experience or electrical schooling Work Environment Mod Physical Demands Regularly required to sit, stand, bend, reach, climb, stoop, kneel, crouch and crawl and move about facility and/or customer's facilities. Frequent lifting up to 50 pounds.  law.


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Discover and use our gifts and talents as "vehicles of His love"
On day 10, the readings turned to pursuit of purpose, discussing calling, career and everything in-between. Let's face it, it's not as easy as it may seem. We grow up thinking we want to be "X" when we grow up and sometimes, many times, life takes us on a different course. As believers, Christians, we know that it is really God who is the conductor of our course. Living life as man directs can cause us to listen first to others and put God's voice a distant second. When it comes to a career, calling or purpose, what has been your guiding life question?
God, what have you made me to do in my generation?
As you consider the aforementioned "what if’s, begin to make a list of your passion(s). What fuels you? What gives you energy?  Passions reflect the beauty of how God made you as well as how HE wants to use you.
If we want to know what God has made you to do, have a mind to know Him and a heart to serve Him.


14 prof steve suranovic fall 2012 problem set #2 - answers answer all of the following questions from the book and.
Answer key to problem set 2 fall 2005 econ 3818-300 1 answer key to problem set 2 assigned tuesday, 7 september due tuesday, 14 september in class: problems 42, 44, 52, 54. Problem set 2 answers: linear programming p dybvig at the start of class next week, submit only problem 3 for grading f. Problem 2 in the funny car competition at the joliet speedway in joliet, illinois in october of 2004, john force complete the â¼-mile dragster race in a record time of 4437 seconds.
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and much more planning news.
01.11.2013 | Our latest planning update keeps you up to date on the major news. This week's looks at the Community Infrastructure Levy Implementation deadline extended by the government, cutting planning red tape is part of the Governments Red Tape Challenge, and much more planning news.
01.11.2013 | Belfast City Airport selects Colliers International to manage retail portfolio
31.10.2013 | An increased willingness to lend by the banks is helping to drive hotel deals throughout the UK, according to the head of hotels at real estate advisors Colliers International.
29.10.2013 | Colliers has all the ghostly credentials needed to market UK’s most Haunted pub.
29.10.2013 former Fat Cat Cafe Bars in central Nottingham and Derby to an established East Midlands operator.
28.10.2013 | Only Two Industrial Plots Remaining At Solstice Park.
25.10further changes to CIL for January 2014, a Community led high street renewal, and much more planning news.
22.09.2013 | Fine-Dining Opportunity at the Compleat Angler in Marlow, on behalf of MacDonald Hotels.
18planning proposals at the recent Government announcement of a joint venture agreement with China.
17.09.2013 | Popular Kent Caravan Park on the Market
16.10.2013 | Michael Hawkins is to joinbased in Manchestery’s 11Government Cabinet reshuffle, Core Strategies and Housing Updates.
08.10.2013 | The world’s premier retail locations have rebounded in 2013 according to Colliers International’s annual Global Retail Highlights report. Whilst New York’s Fifth Avenueshopping street, London’ork’s Madison Avenue.
04both.
30.09.2013 | Laurence Hatchwell OBE is to join Colliers International as a Director within its Rating team based in London.
26.09.once again secured the much coveted award for ‘Office Agency Team of the Year’ at this year’s Yorkshire Property Industry Awards.
25.09.2013 | Colliers to sell Hotel situated at world famous birthplace of British Motorsport
24.09.2013 | Ed Miliband's intention to freeze business rates considered a "sticking plaster"
20.09
09.09.2013 | The Co-operative Estates has completed one million sq ft of industrial property sales in 2013, including disposals and lettings across a portfolio of 18 units.
23.08update looks at decisions on development in the Green Belt, statistics on House building, and proposals for the new Queens Park Rangers stadium.
23.08.2013 |The Old Brewery House Hotel, one of the town’s best known establishments in Reepham, Norfolk.
16
15.08.2013 |.
UK Hotels Sector Heats Up For Investors
13.08.2013 | Colliers International - Hotels has experienced 28 per cent uplift in UK hotel transactional activity in the 12 month period to July 201312.08.2013 | If you think you’ve had a tough week,


The X Files Series 1 - 20. Darkness FsomeLarry Moore Jason Beghe Steve Humphreys Tom O'Rourke Doug Spinney Titus Welliver Bob Perkins Barry Greene Dyer Ken Tremblett Crew Director Chris Carter Writer Joe Napolitanl


i often get asked. If you need any questions answered that are not listed, please feel free to contact me.
My friend passed after 10 lessons so why do I need more?
My Dad says driving lessons are a waste of money and he will teach me to drive.
If I stall the car on my driving test, will I fail?
Can i learn to drive on an international licence?
Will I be charged if I cancel a lesson?
Which is the best method for booking my lessons with you?
There is no truth in this statement, but sometimes those who have failed their practical driving test use this reason to explain why they havenÂ't passed. If you are up to the required standard of a driving test, you will pass. The fact is a practical driving test is a very difficult exam to pass and over half the candidates do not achieve the required standard.
TThis is not a hard and fast rule – some pupils need more lessons than others because everyone learns at a different pace.
The driving test has developed to match the changing conditions on the roads. Traffic is heavier and more congested with new signs and routes to follow. Pupils now have to pass a theory test and the 40 minute practical driving test involves reverse parking manoeuvres, the 'show and tell' section and a 10 minute independent driving section. In previous years, a driving test candidate may only have answered a few Highway Code questions. Older drivers are often the first to admit that they might have difficulty passing a test these days.
If it happens on a normal stretch of road and you deal with it in a safe manner, this neednÂ't count as a major fault and you can still pass your test. If you stall in a dangerous situation and your vehicle becomes a hazard, such as on a roundabout, this may result in a fail.
Not at Heath Sowden Driving School. The aim is to teach you to become a safe and responsible driver and as soon as you are at that level, to apply for your practical driving test. The main cause for people failing their test is that they have taken it before they are ready. This is a false economy as it will result in more lessons, driving tests and overall cost.
In certain circumstances you are permitted to drive for one year before having to take the UK driving test. However if you wish to change to a full UK driving licence, you may have to take the theory and driving test. Please Click here for further details on rules concerning international licences.
No. Piggy backing as it's sometimes called is a way for instructors to cut down on their travelling time between lessons. It is not something that will happen when learning to drive with Heath Sowden Driving School. All lessons are on a one to one basis.
Yes but I would have to sit in the rear of the car and wouldn't be allowed to intervene or comment in any way. Some pupils have found having their driving instructor in the vehicle during their driving test a distraction – others a boost to their confidence. It is entirely an individual's choice.
Currently practical driving tests are £62.00 for tests during weekdays, and £75.00 for tests on weekday evenings and weekends, and can be booked Here.
Currently the theory test is £23, and can be booked Here.
I prefer customers to ring me regarding lessons as you will be guaranteed a quicker response.


shaming a mayor who begged for help after a devastating natural disaster. Of course, Fox News sent Rivera to further politicize this event. He tried to back Cruz into the corner and twist her words, and she was quick to school him over what he was doing.
This should come as no surprise from Rivera. This is the man who once claimed tere at fault because they did not fight back against the shooter.
Featured image via Flickr user Mark Taylor / CC BY 2.0.


The team went into the CIF playoffs with a 26-4 overall record and a 16-game-longgo undefeated in Mission League in 17 years, as they finished with an 18-0 league record.
Compared to last year, players triumphed against old rivals to assert dominance during league play. Last season, the squad won only two games of the three in the series against Alemany and Chaminade and only one out of three against Notre Dame and Loyola. The team swept all its series this year.
These successes on the field that led to undefeated league victory were the result of a dynamic play and cohesive teamwork, first baseman Calvin Koo ’18 said.
“What made those moments after the final out special was seeing everybody jumping around with such pride and heartfelt happiness, not for themselves, but for each of our teammates,” Schreck said.


Clichy
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 New York Times Book Review “One of the best new writers in the field today.
Lynne-marie | 2/9/2014
Frank Mcgeough | 2/7/2014
" a bit disjointed and confusing. somewhat cliche ridden. "
Soho Press | 1/27/2014
" The fifth installment in the Aimee Leduc mystery series, set in Paris. In this book, Aimee is trying to do a favor for a Vietnamese nun at the Buddhist temple where she's learning to meditate when she ends up with a priceless cache of jade that a lot of dangerous people seem to want. I love this book because of the post-colonial themes in it. "
Monty | 1/25/2014
" I really enjoyed reading this book, maybe a little more than the others in the series so far. I think I am getting to know Aimee Leduc better in each book and I am liking how, in spite of her being brilliant, she has so many human frailties. As usual, there are many characters, an evolving story line, poorly matched love life, needing to heal from injuries from the last book, getting new injuries in this book, and more. There is a map of the Clichy district in Paris in the front of the book. I have never been to Paris, but I would think that anyone who has would have fun following the map as the the story enfolds. "
E Goldberg | 1/23/2014
" Another fantastic book in the Aimee Leduc series! Cara Black describes a Paris that is not for tourists or the faint of heart! I can hardly wait to read the next one and I don't have to. "
Clairbp | 1/14/2014
" I want to live in Paris for a year! "
" I love this series. Sure, there are too many things going on and maybe the characters aren't that well developed but I still can't put them down! "
Carol | 1/8/2014
Annette | 12/15/2013
The Library Lady | 11/19/2013
" The one thing I keep asking myself throughout these books--was France really that much more technologically ahead of us in 1994? Laptop computers? Cellphones being fairly commonplace? The use of the Web? "
Val Sanford | 11/7/2013
" Interesting twist pulling forward the history of French Indochina info modern France. But the tangle was too tangled and the story too unbelievable. "
Annie | 5/4/2013
Aingeal Stone | 1/28/2013
Patrinka Selland | 11/1/2012
" Interesting. not great, but somehow I can't stop reading the series. this is the 5th book so far all based in various neighborhoods of paris. "
Kara | 3/5/2012
" Good summer mystery - love the Paris setting. "
Concetta | 2/2/2012
" Loved it! Another murder mystery in Paris. I could see, taste, hear and smell everywhere Aimee went. C. Black knows how to weave a mystery that keeps my attention. I had to finish reading this one in my car in the park/ride this evening before I could drive home! "
Nadia | 1/13/2012
" Aimee Leduc is a private investigator in Paris. Each of the books focuses on a different neighborhood of Paris. The "crimes" and investigation are very contemporary and sometimes brutal. Not for the weak-kneed mystery reader. "
Sara | 5/13/2011
" Another great story. I love the Paris locale. "
Catherine Woodman | 12/31/2010
" Aimee Leduc is a complex character who almost seems to swirl in these books--they are very atmospherically written and wonderful to read "
" Great setting - Paris. Weak writing....repetition of descriptions, sporadic development of story line. I will however read another book in this series. Hmmmm. "
Remy | 7/15/2010
Concetta | 5/27/2010
The Library Lady | 9/12/2009
E | 4/7/2009
Kim | 12/15/2008
" A quick read, entertaining and very evocative of Paris. "
Linda | 9/17/2008
" Another page turner. I'm enjoying this series. I've decided to suspend my technology disbelief. "
Annie | 8/19/2008


The first round of the Bouldering World Cup will take place this weekend at Erlangen in Germany. There will be live coverage on the web here. Gaz Parry will also be reporting on the event on his Blog. Nick Sellars has made auth (source: UKC) According to 8a.nu American youth Paul Robinson has climbed 100 boulder problems graded Font 8A or harder in a year, and 6 graded 8B or harder in a week! Dave MacLeod has established yet another new hard boulder problem at Dumbarton Rock. Sosho takes in 18 metres of roof climbing and weighs in at Font 8A+. Check out the report and photos on his Blog.


Mayor Healy’s Jersey City Dream Plan
Maps of the future bike plans were posted on a tri-fold canvas next to the presentation podium shared for all to view. After all speakers commented there was a premier of the city’s safe cycling awareness PSA presented to everyone via projector (link posted at the bottom of page) and then concluded with an extremely brief Q&A hosted by Healy.
All and all, this is a very positive movement in a much-needed area, steps in the right direction. We at Grove are all very excited to see these changes begin to be implemented and are all in favor of the movement.


Before the vote, Len McCluskey, leader of the Unite union – a big financial donor to Labour and supporter of Jeremy Corbyn in internal political battles – wrote to MPs urging them to support Heathrow expansion.
These claims are obviously dodgy.
■ Red Green Study Group submission to Labour policy consultation
■ “Til they have built a third runway” – Richard Seymour
■ “Heathrow: step up the campaign” – Alan Thornett, with links to other information at the end
■ People and Nature site contents


 To make sure everyone can join in the fun, most of the equipment is ideal for children with disabilities.
Keen to brush up on your table tennis skills? Outdoor tables are available around the play areas – free of charge, just bring along some bats and a ball.
You will also be able to testing our your skills out at the new skate park in Oxhey Activity Park, which has a good mix of street obstacles, as well as a wide mini ramp if you’re after some air time. The park includes a bike pump track and smooth skate run, so people of all ages and abilities can work on their wheel skills.
A jump box section for the younger bike and scooter enthusiasts will enable them to try out jumps and aerial tricks too. Plus, a separate learners’ skate park next to the café will give absolute beginners the perfect spot to start honing their skills before they progress to other parts of the park.


The principle of integration which underpinned Second Wave civilization and led to mass production and its concomitants of synchronization, standardon, is the very principle that comes under attack in Third Wave Civilization and leads to the breakdown of industrial systems and "indust-reality" (110) Indust-reality is Toffler's word for a worldview based on machine technology that brought with it "a redefinition of God...of justice...of love...of power...of beauty ...new ideas, attitudes and analogies. ...
sources – the teacher, the priest, the chief or official and, above all, the family ...
Techno-rebels, people who see the technological threat to life on earth, ...


After four largely productive seasons on the North Side for the String Bean Slinger, the Cubs lost patience with Edwards last summer after an awful 20 appearances with an 8.47 ERA (5.87 FIP) and an injury to his non-throwing shoulder, sending him to San Diego for Brad Wieck.
Edwards only appeared in two games for the Padres, giving up six runs in 1 2/3 innings. He became a free agent on Nov. 4.
Edwa(!) pick of the Rangers in the 2011 draft. Two years later, he was traded to the Cubs along with Neil Ramirez, Justin Grimm and Mike Olt in exchange for Matt Garza. He is missed.
2. Kendall Graveman.
Graveman is kind of this year's Drew Smyly.
The Cubs took a flyer on the former A, who was coming off Tommy John surgery. He never made it past two minor league rehab starts in September. It would have cost the Cubs $3 million to retain him; they declined and the Mariners, fresh off their Carl Edwards signing, swooped in with a one-year, $1.5 million deal. He is not missed.
3. Pierce Johnson.
Not sure why that wasn't good enough for Hansin, but Johnson was a real disappointment for the Cubs, who made him their first round pick in 2012. He pitched all of one inning for the big league club (giving up). He is not missed.


probably know Cand replaced it with a new piece of legislation. No Child Left Behind has been a spot of contention with parents, teachers, and politicians over the last 14 years, so this is a good thing, right?


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NA negative hour angle means the Sun hasn't been in the south yet, this day. The angle -10 degrees is of course the same as 350 degrees, i.e. adding or subtracting even multiples of 360 degrees does not change the angle.
We compute sin(h), and then take the arcsine of this to get h, the Sun's altitude above the horizon.
2. Computing the Sun's rise/set times.
As you can see, there are several altitides to choose among. In most countries an altitude of -0.833 degrees is used to compute sunrise/set times (Sun's u). One exception is the Swedish national alamancs, which use -0.583 degrees (C - however my own Swedish almanac Stjärnhimlen ("The Starry Sky") uses the international convention of -0.833 degrees.
If cos(LHA) is less than -1.0, then the Sun is always above our altitude limit. If we were computing rise/set times, the Sun is then aboute the horizon continuously; we have Midnight Sun. Or, if we computed a twilight, then the sky never gets dark (a good example is Stockholm, Sweden, at midsummer midnight: the Sun then only reaches about 7 degrees below the horizon: there will be civil twilight, but never nautical or astronomical twilight, at midsummer in Stockholm).
If cos(LHA) is greater than +1.0, then the Sun is always below our altitude limit. One example is midwinter in the arctics, when the Sun never gets above the horizon.
Now, if we add LHA to UT_Sun_in_south, we get the time of sunset. If we subtract LHA from UT_Sun_in_south, we get the time of sunrise.
c) Iterate b) until the computed sunrise or sunset no longer changes significantly. Usually 2 iterations are enough, in rare cases 3 or 4 iterations may be needed.
This is really done the same way as the Sun's rise/set times, the only difference being that you should compute the RA and Decl for the Moon and not for the Sun. However, the Moon moves quickly and its rise/set times may change one or even two hours from one day to the next. If you don't iterate the Moon's rise/set times, you may get results which are in error by up to an hour, or more.
Since the Moon rises and sets on the average 50 minutes later each day, there usually will be one day each month when the Moon never rises, and another day when it never sets. You must have this in mind when iterating your rise/set times, otherwise your program may easily get caught into an infinite loop when it tries to force e.g. a rise time between 00:00 and 24:00 local time on a day when the Moon never rises.
Yes, computing the Moon's rise/set times is unfortunately messy, much due to its quick orbital motion around the Earth.
a) Compute the RA and Decl for that body instead of for the Sun. If the body is a star, get its RA and Decl from a suitable star catalog.
b) GMST0 is still needed, so you should compute the Sun's mean longitude.
Since the planets move much slower than the Moon, and the stars hardly move at all, one need not iterate. If one wants high accuracy, one may find it worthwhile to iterate the rise/set times for Mercury, Venus and Mars (these are the planets that move most quickly).


Today we have the gift of hindsight, knowing what would eventually happen to Szalasi and his movement, but at the time he did not know where this radicalism would end. Undoubtedly he must have suspected that his dark vision was headed towards the abyss. While ensconced in Velem, pondering what looked like the inevitable, what did Szalasi do?Did he scale the 582 meter St. Vid Hill and gaze out across the snow covered landscape, reflecting on what was happening to him and the insidious movement he led?Did he beg repentance at the St. Vitus Church in Velem?


Hello, loves! I'm in a good mood today because of these cuties! Were did I got them? Since I am stuck at home, I go shopping online. (I miss the malls, huhu) And one day I received a notification from a one-day P11-sale on Shopee. Yes, selected items were on sale for a day and … Continue reading 11-peso washi? Yes please!


Wales Heart Research Institutedom
Analysis of the intracellular organization of RyR particles using freeze-fractured, negatively stained muscle SR junctions, revealed that RyRs are packed into discrete, two-dimensional arrays termed couplons (1922). In recent studies using RyR1 purified from rabbit muscle SR, the assembly of RyRs into a regular, chessboard-like two-dimensional array was found to be an intrinsic property of RyR oligomers and not dependent upon insertion in the SR membrane or ancillary proteins (23). The projection maps derived from these reconstituted RyR arrays resembled those described previously for junctional SR arrays observed in situ (23). Thus, RyR molecules associate together to form an ordered array, enabling individual oligomers to interact allosterically, creating an integrated microdomain structure within the SR membrane. The RyR-RyR interaction site is found within the cytoplasmic domain (23), which is highly conserved in mammalian and non-mammalian species (49) in regard to topology and size (14, 24), suggestive of an essential functional role (25). RyR array formation is a shared feature of the distinct isoforms because both RyR1 and RyR3, when transfected and expressed independently in a dyspedic myotube cell line, also formed similar two-dimensional arrays in situ (26, 27).
The concept of the couplon, comprising arrays of interacting RyRs (and ancillary proteins such as calmodulin, sorcin, and FK506-binding protein (FKBP12)) (20, 21), provides a model to explore the underlying structure and function relationship. The lateral dimensions of individual couplons and their distribution within the myocytes vary with muscle type (22) and may influence the kinetics of local and global Ca2+ signals. Ca2+ sparks originating from the SR were first observed in cardiac myocytes using fluo-3 confocal microscopy (28). However, it remains unclear whether a spark emanates from a finite structural entity, i.e. it equates to activation of an entire couplon, or whether it involves a fraction of the RyRs. The extent of recruitment could depend upon both the mechanisms initiating the spark and the regulatory influences modulating RyR activity (29, 30), including allosteric interactions and the disposition of other proteins (31). In cardiac muscle, the "local control" model of Ca2+ signaling ("cluster bomb" hypothesis) postulates that a small number of RyR channels interact spatially with each L-type voltage-operated Ca2+ channel (dihydropyridine receptor), and then calcium-induced calcium release operates to trigger the opening of other RyRs (20, 3235). Combined patch clamp and confocal measurement of Ca2+ in the subsarcolemmal space estimates that each SR Ca2+ release event ("spark") requires the concerted opening of four to six RyR molecules, following Ca2+ influx via one dihydropyridine receptor in isolated cardiac myocytes (36). The mechanism of simultaneous activation of the RyR molecules within the cluster to produce a Ca2+ spark remains uncertain. Stochastic gating theory suggests that each RyR molecule activates and closes independently (20, 33). An alternative hypothesis proposes coupled gating, involving the functional interaction of adjacent RyR molecules, mediated by FKBP12 or 12.6, as demonstrated for RyR1 and RyR2 channels recorded in planar bilayer experiments, respectively (37, 38). Similar results with coupled gating of inositol trisphosphate receptors (39) suggest that this may be a common mechanism for related Ca2+ release channels. Thus, RyR-RyR oligomeric interaction may underlie a central mechanism for regulation of EC coupling insites of interaction between RyR oligomers. The strategy used was to trypsinize RyR and detect liberated peptides that specifically bound to immobilized tetramers of RyR, using site-specific antibodies for different regions of the RyR. A putative binding region was identified using this approach. The corresponding RyR protein fragments were expressed in vitro and as GST fusion proteins and then tested for binding to RyR by immunoprecipitation, ELISA, and GST pull-down assays. The results describe a potential RyR binding region near the center of the linear RyR sequence, within which were a number of potential binding domains.
Preparation of hRyR2 Expression Vector ConstructsThe coordinates of the expression vector constructs, corresponding to the protein sequence of human RyR2 (hRyR2), are given in Figs. 2A and 3A (6to contain appropriate restriction sites in-frame with the vectors used and were synthesized by Sigma Genosys. RyR constructs 1, 2, and 3 were inserted into a pGBKT7 vector (Clontech) for in vitro expression. For RyR construct 1 the primers were 5'-AAGGATCCACGCTTCTCTCATTGAC-3' (forward) and 5'-AGGACTCGAGTTCCAGGTCCTTAAA-3' (reverse); for RyR construct 2, 5'-CCGGATCCATAATATATGGCCAAAG-3' (forward) and 5'-CTGGCTCGAGTTTCTCCAAAG-3' (reverse); and for RyR construct 3, 5'-AGGGATCCTTAACCCACAACCT-3' (forward) and 5'-CCTGCTCGAGGTTTTCCATGGT-3' (reverse). DNA constructs encoding GST fusion proteins were made using pGEX vectors (Amersham Biosciences). GST-RyR 4, 4A, 4B, and 5 were constructed in pGEX-5X-2, whereas GST-RyR 6 used pGEX-4T-3. For GST-RyR 4 the forward primer was that used for GST-RyR construct 1, and the reverse was 5'-CAGCGTCGACAGAAACCTGGCT-3'. For GST-RyR construct 4A the forward primer was that used for RyR construct 1, and the reverse primer was 5'-GAGGCTCGAGTGTTTTTCCATCATATCGACATA-3'. For GST-RyR construct 4B the forward primer was 5'-CGCGGGATCCAGTCATCAATGGATTCTGAA-3', and the reverse primer was that used for GST-RyR construct 4. For GST-RyR 5 the primers were 5'-CCGGATCCTTGACATGAGCAATGTTA-3' (forward) and 5'-ACATGTCGACTGAGTTGTTGGAGGAAAC-3' (reverse). For GST-RyR construct 6 the forward primer was 5'-CTATGGATCCGTGATGAAGACTGGCCTGGAG-3', and the reverse primer was that used for RyR construct 2. All constructs were generated by PCR using Pfu DNA polymerase (Promega) and full-length hRyR2 DNA as the template (6) and were confirmed by DNA sequencing (ABI PRISM Big Dye kit; Applied Biosystems).
Preparation of GST-RyR FragmentsThe protocol was based on the manufacturer's instructions for pGEX vectors (Amersham Biosciences). GST-RyR fusion protein expression in bacterial cultures (1l, 25 °C, Escherichia coli BL21 Rosetta; Novagen) was induced by 0.1 mM isopropyl -D-1-thiogalactopyranoside when the absorbance (A600) reached 0.5. Cells were harvested after 3 h and the pellets frozen at 80 °C until required for purification. The batch purification protocol with glutathione-Sepharose 4B beads and elution with reduced glutathione was used (Amersham Bioscience). Reduced glutathione was removed by overnight dialysis of the eluted recombinant protein against PBS, pH 7.4. Purified GST fusion proteins were stored at 80 °C until use.
Binding of RyR Tryptic Fragments to Immobilized RyRSolubilized RyR from skeletal muscle microsome23). To lower the NaCl concentration to 200 mM, the RyR peak fraction was diluted with 20 mM PIPES buffer, pH 7.1, 0.5% CHAPS, 100 µM EGTA, 150 mM CaCl2, 2 mM DTT, and protease inhibitors (0.2 mM AEBSF, 0.5 mM benzamidine, 1 mM iodoacetamide) and concentrated to 1.5 mg/ml protein using centrifugal concentrators (Amicon). Protease inhibitors were omitted from gradients when used for trypsinization experiments, and DTT was omitted from preparations used to couple RyR1 to agarose. Trypsin digestion was performed in200 mM NaCl, with 800 µg of RyR protein and 2 µg of trypsin (protein:trypsin ratio 400:1 w/w (40)). At specific intervals up to 30 min, trypsin digests were stopped by the addition of 100 µg of trypsin inhibitor and AEBSF to 0.2 mM.
Preparation of Enriched RyR1Native RyR1 was prepared from solubilized skeletal muscle microsomes (23) using fast protein liquid chromatography anion exchange chromatography with a 20-ml Resource Q column (Bio-Rad) in place of sucrose gradient centrifugation. RyR1 was eluted with a 1251,000 mM NaCl gradient0.2 mM AEBSF, 0.5 mM benzamidine, and 1 mM iodoacetamide. The RyR protein peak eluting at 350 mM NaCl provided the RyR1 preparation used for all experiments other than those with trypsin digests. The protein concentration in this fraction was typically 1 mg/ml. In all of the experimental protocols described, the final NaCl concentration was calculated to allow for the 350 mM NaCl contribution of the buffer containing the enriched RyR preparation.
RyR Interaction ELISAsEither 10 µg of RyR1-enriched preparation or BSA was added to the wells of a 96-well ELISA plate (ICN) for 2hat22 °C in PBS and bicarbonate buffer (41). The wells were washed three times with PBS. 100 µl of GST-RyR fusion protein (1,000 ng/well), made up in assay buffer, 20 mM PIPES, pH 7.1, 0.5% CHAPS, 1% BSA, and 150 mM NaCl (unless otherwise indicated) was added and the plates incubated on a rocker at 4 °C, overnight. This buffer contained 1% BSA to block nonspecific binding and was used to maintain the specific binding conditions for the duration of the assay (20 h), including the addition of Abs. The wells were washed three times, and anti-GST Ab (1:10,000 dilution) was added for 1 h at 22 °C. The wells were washed three times, and secondary Ab (1:10,000 dilution) was incubated at 22 °C for 1 h. After four final washes with PBS, SigmaFASTTM OPD peroxidase substrate was added, and the reaction was stopped after 30 min with 3 N HCl and A495 determined using a plate reader.
GST-RyR Fusion Protein Pull-down Assays of RyRThe studies described above demonstrate that interactions between intact RyR and smaller RyR fragments, expressed either by an in vitro expression system or as GST fusion proteins, can be monitored by immunoprecipitation or a sandwich ELISA, respectively. With each of these techniques, the RyR fragment was the assay component detected, i.e. via [35S]methionine incorporation or via GST tag detection. To examine whether the component within RyR preparations specifically interacting with the fragments was RyR itself, GST-RyR fusion protein pull-down assays were performed and the pellets analyzed for presence of the native RyR. Fig. 5A shows Coomassie-stained gels of the enriched RyR1 (lane A) and RyR2 (lane B) preparation Fig. 5B shows Western blots for RyR1 and RyR2 isoforms after incubation with GST-RyR fusion proteins, se 4B beads. The two panels of Fig. 5B illustrate the RyR1 and RyR2 protein, migrating at 560 kDa, detected within the pellet after GST-RyR pull-down. Fig. 5B (upper panel) shows that RyR1 binding was observed to both GST-RyR 4 and 6 and the control protein GST-FKBP12, but that no interaction was detected with GST-RyR 4A and 5, nor with the GST moiety alone. Similarly, RyR2 also exhibited binding to GST-RyR 4 and 6 (Fig. 5B, lower panel) and to GST-RyR 5. As for RyR1, no interaction was found between RyR2 and GST-RyR 4A nor GST alone. These results confirm the detection of a valid association between native, solubilized RyR and specific RyR fragments expressed in both bacteria and in mammalian cell systems.
The proteins precipitated by GST pull-down assays were subjected to silver stain analysis. Fig. 5D shows the proteins detected after pull-down assays with GST, and GST-RyR constructs 4 and 6 analyzed by 5.5% or 15% polyacrylamide gels, to illustrate higher or lower molecular mass proteins, respectively. Both gels show a band around 66 kDa, which corresponds to the BSA used to block nonspecific binding and which is present in all lanes except that of the enriched RyR preparation, where it was omitted for clarity. Another prominent band was observed in the 5.5% gel at about 100 kDa and also appeared in all lanes. These bands were therefore nonspecifically bound to the beads. The only other band apparent in the 5.5% gel is close to the top of the gel with a mobility corresponding to the RyR and can be discerned only in lanes 4 and 6, but not in the GST lane. The 15% gel confirms the presence of GST fusion protein bands corresponding to the appropriate molecular masses shown in the Western blots in Fig. 3C.
RyR functions as a homotetramer, and the four subunits remain stably bound in a complex upon solubilization of the RyR from SR membranes using high ionic strength buffers and CHAPS detergent (48). Analysis of the RyR homotetramer topology reveals the RyR molecule to be highly folded (14, 15). Thus, there may be a variety of interacting regions within the RyR molecule which maintain both the intrasubunit tertiary structure and intersubunit associations binding the tetrameric complex together. To minimize identification of intramolecular and intersubunit binding regions and to enable a specific focus on the surface-exposed areas where RyR-RyR interoligomer binding domains would be situated, our experimental rationale was to employ the native RyR tetramer as the interactant in disparate binding interaction assays.
In our initial study, we generated RyR fragments from enriched RyR1 preparations by typsinization and mixed the resultant fragments with RyR tetramers bound to a solid support. These binding studies were performed using 200 mM NaCl because regular two-dimensional arrays of RyR were formed only at concentrations below 250 mM (23). Using epitope-specific RyR antibodies, only a 25-kDa tryptic fragment detected by Ab34 was identified in the immobilized RyR1 column eluate, suggesting that this specific region was able to bind directly to the intact RyR oligomer (Fig. 1). The pattern of proteolytic cleavage monitored by RyR antibodies suggested that the RyR N terminus was extremely vulnerable to trypsin because Ab2142-immunoreactive bands were removed most rapidly within 30 min. With Ab34, the immunoreactive bands were similar at 5 and 15 min and, although diminished, these were still visible after 30 min. The C terminus appeared to degrade slowly, with the Ab2160-reactive triplet consistently observed from 5 to 30 min, with gradual accumulation of a <32 kDa band. These data agree with previous work where proteolysis of SR proteins detected with site-directed RyR Abs (49) indicated a protected RyR C terminus, consistent with colocation of a hydrophobic region comprising the predicted RyR transmembrane domain that is SR membrane-embedded. This C terminus protection apparent with our solubilized RyR1 preparations indicates that this region may be buried within the protein or that the detergent annulus confers a similar protective effect. A previous RyR proteolysis study identified a 33-kDa fragment, involving trypsin cleavage at Arg-2840 and Arg-3119 (50), similar to the 25-kDa fragment detected by Ab34 in our studies. This central region of RyR also coincides with a cleavage-sensitive zone demonstrated with trypsin (49) and also with Lys-C and Glu-C proteases (51) and has thus been interpreted as being surface-exposed. Thus, our data are consistent with that of previous reports in identifying a region predicted to have a surface topology in the RyR complex and is therefore a good candidate region for one site participating in the RyR-RyR oligomericgions of RyR involved in oligomeric interactions that are outside of the limited range of the epitope-specific antibodies used in this study.
The ELISA also enabled a profile of the ionic strength dependence of GST-RyR fusion protein binding. GST-RyR fusion proteins 4 and 5 bound optimally in the range of 50100 mM NaCl, whereas for GST-RyR 6 maximal binding was shifted to 150450 mM NaCl. Binding of these same constructs to RyR2 at 150 and 450 mM NaCl showed the same pattern of binding for the GST-RyR constructs. Previous in vitro RyR-RyR interactions observed with purified RyR were not retained above 250 mM NaCl, indicating that high ionic strength buffers may be disrupting an electrostatic interaction between RyR molecules (23), entirely consistent with requirement of 1 M NaCl for efficient RyR solubilization from native membranes (48). The results for GST-RyR fusion proteins 4 and 5 support this idea, but the binding profile for GST-RyR 6 suggests that other types of interaction may also be important. In silico analysis of the RyR sequences in the candidate binding region revealed three regions with probability of a coil secondary structure (Fig. 6). In the classical coil, hydrophobic amino acids reside in the a and d positions of a 7-amino acid stretch of an -helix (labeled ag) called a heptad, which is often repeated and which provides the coil backbone (44). The specificity of interaction is provided by key amino acids at other positions in the heptad repeat, particularly the e and g positions, and these are often charged (52, 53). The COILS server identified a strong coil probability of a 28-amino acid stretch of four heptad repeats in GST-RyR 6 (Table I). The coil probability within repeat region 3 (GST-RyR 5) was more variable and dependent upon the weighting for a and d amino acids and charged amino acids at e and g (Table I), despite the close sequence homology between the isoforms. The maximum binding of GST-RyR 6 at high salt concentration and the shoulder at 450 mM NaCl for GST-RyR 5 suggest a hydrophobic component to the binding mechanism. Coiled-coil interactions are often quite complex and are not always defined by a 7-amino acid heptad repeat; amino acid stutters and staggers in between heptads have been reported, and 11 amino acid sequences have also been observed (52, 53). Charged amino acids play a role in establishing and maintaining the coiled coil interaction and in its specificity (53). In our experiments, the presence of the GST moiety in the RyR fusion proteins enhance solubility and ease of purification but may also influence the response to NaCl. An additional consideration is that our data do not only reflect the binding properties of the fusion protein, but also of the native RyR complex used as the binding partner. Evidence for an -helix secondary structure was displayed by all of these regions when examined on the Sspro (54) and Impred servers (43), and the latter also identified coil 3 in GST-RyR 6 (Table I and Fig. 6). A limitation of our experiments is that they do not address whether the potential binding domains in this region interact with themselves or with a distinct complementary binding region of the RyR, as illustrated in Fig. 6C. Adjacent RyR molecules within an array should interact with their protein sequences running in opposite directions, and thus any involvement of coiled coils would have to be antiparallel (55). The in silico data provide potential insights into the mechanism involved in RyR-RyR interaction but require further investigation.
The coils correspond to the hRyR sequences shown diagramatically in Fig. 6B. The coordinates are given as the first and last three amino acids in the corresponding hRyR2 sequence. The maximum value for probability of coil participation assigned to corresponding sequences for RyR1, 2, and 3 is listed. Wt and UnWt, respectively, refer to whether the COILS program was weighted for hydrophobic residues in the a and d positions and charged amino acids in positions e and g of a heptad repeat or not. See "Experimental Procedures."
It has been implicit throughout these experiments that binding of RyR2 constructs to RyR1 tetramers was legitimate as an experimental approach. This was considered reasonable at the outset because the characteristics of both physical interaction in situ and functionally with coupled gating in vitro were observed for both isoforms (37, 38). Moreover, the sequence of all three RyR isoforms in this central region is highly conserved. To address this issue experimentally, ELISAs and the GST pull-down assays were performed with both RyR1 and RyR2 preparations. GST-RyR 4 and 6 captured both RyR1 and RyR2, and, in addition, RyR2 was precipitated by GST-RyR 5. In general, interactions with GST-RyR 5 were weaker and showed inconsistencies, and in the GST pull-down assays there were differences in the proportionate quantities of each RyR isoform captured by each construct. This might suggest that the interaction of GST-RyR 5 for RyR2 was better than with RyR1, as control experiments were consistent for both RyR1 and RyR2 isoforms. Taken as a whole, the data suggest that using native RyR1 for interaction assays with RyR2 fragments provides a stringent test for binding regions that are conserved between RyR isoforms and therefore unlikely to produce a false positive.
The enriched RyR preparations contained a number of other proteins. Toin nonspecific binding to the GST fusion proteins, the size of the interacting protein was determined. The enriched RyR preparation was separated on a native acrylamide gel to minimize disruption of secondary and tertiary protein structure. It was however necessary to treat the preparation first with zwittergent to separate the RyR into monomers because the tetramer (2,300 kDa) was too large to enter the gel. Overlay assays with GST fusion proteins (Fig. 5C) showed that the GST-FKBP12 fusion protein only associated with the RyR band, thus confirming the validity of the overlay protocol. Similarly, the GST-RyR fusion proteins 4 and 6 also bound strongly to the RyR band. Running native gels often leaves protein complexes intact, so it was possible that another protein acted as a intermediary between the RyR and fusion protein. However, treating the enriched RyR preparation with zwittergent, which breaks the association between RyR subunits, probably destroyed any ancillary protein binding also. Furthermore, any ancillary proteins would be present as lower molecular mass contaminants, and no binding of fusion proteins was seen in this region. Finally, the silver-stained gels (Fig. 5D) showed only one major contaminant at about 100 kDa, which was probably the SR Ca2+-ATPase (10). This, however, was present in the recombinant GST control lane and was possibly binding the glutathione-Sepharose 4B beads used for the pull-down assay. Thus, although none of the different binding techniques used was singularly definitive, the combined data from the variety of approaches employed consistently suggest that RyR and GST-RyR fusion proteins 4 and 6 (and possibly 5) interact in a specific fashion.evidence for the Ab34 binding region of the RyR (hRyR2 25403207) as a likely participant in the RyR-RyR oligomeric interaction. Recombinant constructs corresponding to this region, expressed in both a mammalian expression system and as bacterial fusion proteins, demonstrate specific binding to enriched preparations of RyR1 and RyR2 homotetramers, respectively. In pull-down assays, both RyR2 and RyR1 are captured by the GST-RyR fusion proteins. The binding was dependent upon the NaCl concentration in the binding buffer for some GST-RyR fusion proteins. Those constructs demonstrating binding at a relatively high NaCl concentration were shown by in silico analysis to contain a coil secondary structure, which would favor the hydrophobic interaction of a coiled coil backbone. Moreover, in independent studies, this central RyR region has been shown to be surface-exposed (49, 51)is region binds to itself or whether other parts of the RyR molecule participate as a complementary binding reguk
1Ab, antibodyBKBP12, FK506hRyR, human ryanodine receptor;RyR, ryanodine receptor.


Adjunctions and comonads in differential algebra
William Francis Keigher
Vol. 59 (1975), No. 1, 99–112
DOI: 10.2140/pjm.1975.59.99
Primary: 12H05
Received: 22 August 1974
Revised: 20 February 1975


Endava PLC Sponsored ADR (DAVA) SurDAVA - Free Report) shares rallied 3.1% in the last trading session to close at $138AV.


Description : B --Find out in this essay. -- An excerpt from Lisa Gardner's Love You More. --Or something worse?
how to live by Sarah Bakewell....


Palmetto’s Manatee School For Arts Has Just Hired Heavily Armed Combat Vets To ‘Put Down’ Active Shooters
February 14, 2019 Alex Hall 299 Views 0 Comments Parkland school shooting, PUBLIC SCHOOLS, school shootings, Second Amendment
Not only is it great for the security of the school, but it also gives our veterans some meaningful employment and a chance to sManatee School for Arts Principal Bill Jones explained that his school is not interested in trying to “talk” or “negotiate” with potential threats — they’re just going to take them out told the paperhe administration has also mandated that the guardians use ammo for the long-guns that does not “over-penetrate,” specifying shells that will pierce basic body armor but not go through the intruder, which would pose a risk to others.
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Why are we here? Simply, Japan is the 2nd largest unreached people group in the world. (JoshuaProject.net)
Read this amazing true story of an atheist who recently become a follow of Christ.
Watch a great message from my twin brother, Doug.
I know that I need better and deeper relationships with my fellow brothers and sisters.  Watching, I realized that this is where my spiritual health is lacking the most.
Our Recent Adventures in Parenthood
Japan's Message: "Have More Babies!"
After hearing Nancy Pelosi's comments, I think we Americans
need to hear this message.
Kurt Warner:  The true story is better than the "movie-version" e-mail!
Kurt Warner grew up in Cedar Rapids, Iowa, went on to my alma mater (The University of Northern Iowa) and became a Christian.  Other than those three similarities the path of this 3-time Super Bowl starting quarterback and mine are very different!  His story is much more exciting.  Read the partly-true e-mail version, and then the even better true story here.
You know who I'll be rooting for during the Super Bowl!
What Sanctity of Life Comes Down To
With Obama's crazy views reversing good abortion laws, we need to turn our focus back on what is at the root of this evil.
"Hemant Mehta, the 'friendly atheist' who gained notoriety by selling his soul on e-bay, asked me if I would answer questions submitted by his atheist friends. I said I’d be glad to."  Here are the questions and Lee's answers.
Posted by mark wolter at 11:36 AM No comments: Links to this post
Here is a recent message by John Piper about abortion translated into Japanese.  I encourage you to read and pass it on!
Posted by mark wolter at 11:14 PM No comments: Links to this post
Do you have any extra Bibles?
"Research indicates that the average American Christian owns nine Bibles and is actively in the market for more. That statistic troubles us here at CRI, since we receive more than 400 letters a month from pastors and Christian workers in developing countries whose churches own no Bibles or Christian books."
Why not consider giving them to people who really need them!  The process is VERY easy.
Posted by mark wolter at 3:51 PM No comments: Links to this post
Darwin's Personal Struggle and the Church
This year, being the 200th birthday of Darwin and the 150th anniversary of the publication of his infamous book On the Origin of Species, I want to devote some time to thinking about Darwin's impact on the world as well as to devote some time to thinking about what impacted him.
Did you know that the idea that the Earth is billions of years old did not come from Darwin?  This thought was deeply entrenched, not just in men of science, but regrettably even in the church long before Darwin came onto the scene.
"The doctor tries to look away as he says, 'I'm sorry.  I've done all I can.  Your daughter is dying.'"
How would you respond?  You see your 10-year-old daughter suffer terribly for several weeks and then you hear the news.  Not being trained well by his Christian culture or by his own church in the book of Genesis, Darwin is pushed over the brink.  "As far as we know, it made him turn away from God once and for all."
"Without the Bible's explanation that Adam's sin brought death and a curse on the world, there is no satisfying answer to why evil exists on Earth.  The church failed to give Darwin a satisfying answer for the sight of the snake devouring a baby bird or the pain of losing his daughter.  Those in the church who claimed that God brought Adam's 'very good' world into existence through millions of years of death and suffering made God out to be an ogre and a liar."
What do you think?  Could God call the created universe "very good" if he used millions or years of death and suffering to do it?  Or is it better to assume that all creatures were created in a literal six-day period and then given the label "very good" (Genesis 1:31) before death had entered the world?  And probably most importantly, are we missing a key part of the gospel when we leave out the extent to which Adam's sin affected world?  How will future sufferers react to a God that casually allows death and tragedy into the world before sin, while counting it all very good?
(Quotes taken from Answer Magazine, p. 33 Jan.-Mar. 2009 edition)
Posted by mark wolter at 10:02 PM No comments: Links to this post
The Secularization of the West
No one has to convince you of it.  While many Americans might becoming more devout Christians, at the same time many more are losing their faith in the Bible.
Could this be a big part of the cause?
Posted by mark wolter at 4:26 PM No comments: Links to this post
Some Things I Love about Japan
A few weeks ago I commented on some things that I thought were strange or unusual in Japanese culture.  Today I wanted to comment on some great things about Japan and Japanese culture.
10.  Cakes are much more lightly sweetened and therefore more distinctly tasteful.  Instead of just getting a mouth full of sugar, like you get with American cake, you are enabled to taste the various ingredients more distinctly.  It took me awhile to get used to it, but once I did, I don't ever want to go back!
9.  While I am on the dessert topic, I love the little spoons and forks that Japanese use.  It makes the whole experience of eating dessert more of a savoring and relaxing time rather than a woofing down and pigging out time!
8.  Japanese toilets school American toilets!  You can set your flow to a #1 or #2 (Depending on your job!) and you can wash your hands at the top with the incoming clean water.  What more could you want!  Of course the plug-in warm toilet seat is a nice addition as well.
7.  Japanese refrigerators are so much better.  More cost-efficient, compartmentalized and easier on the environment.
6.  Cozy houses, not hotels.
5.  Polite waitresses and waiters - especially to foreigners!
4.  Most people are thin and eat appropriate portions.  Restaurant food is good, healthy, and it is always just the right amount.  (OK I admit, I'm not a big eater.)
3.  Though most Japanese aren't very direct, they are sincere and trustworthy.  You don't get a lot of smart-alecks or blatant liars here.
2.  Japanese Christians are so precious.  They persevere under extremely dry and spiritually dead environments, face social ostracation and yet manage to do a lot of great work for the kingdom, even in their busy work-filled lives.
1.  Of course number one is my wife Maki.  Without her wonderful presence in my life, Japan would indeed be a very difficult place for me to stay long-term!  I love my family and I love my homeland too much.  God knows!
Posted by mark wolter at 8:26 PM No comments: Links to this post
Some have asked why we chose the middle name of our new son.  Our son is named after a man I respect very much and would like my son to emulate in his own life as he lives to love God and others in all the nations.
Here is a summary from a post I made last May:
Adoniram Judson was the first American overseas missionary. His testimony is a greatly inspiring one (you can read or listen to it here.)
After leaving for Burma (Myanmar) his first two children died in infancy and then his wife died. Six months later his third son, age 2, also died. Many would have understandably given up and gone home. Not Judson. He stayed on for 38 years until he died at the age of 61 in Burma. When Judson was lying loaded with chains in a Burmese dungeon at one point, a fellow prisoner asked with a sneer about the prospect for the conversion of the heathen. Judson calmly answered, "The prospects are as bright as are the promises of God."
Judson eventually saw God break through. He was remarried and had other children. And by faith in the promises of God, God mercifully allowed him to see some amazing fruit. Hundreds were saved from eternal destruction in hell and God showed Himself MIGHTY to save. It is the glory of trusting the "impossible". In 2003 Patrick Johnstone estimated that in Myanmar the Baptist Convention (from which Judson came) to be 3,700 congregations with 617,781 members and 1,900,000 affiliates- this is due largely to the fruit of this dead seed who trusted in a God who can do the impossible.
May we all live like Judson and as Martin Luther said, "Let goods and kindred go, this mortal life also. The body, they may kill. God's truth abides still. His kingdom is forever!" or as Hebrews 13:13 says, "Therefore let us go to him outside the camp and bear the reproach he endured. For here we have no lasting city, but we seek a city that is to come."
Posted by mark wolter at 7:48 PM 1 comment: Links to this post
3 Myths about Calvin
I've been reading Calvin's Institutes of the Christian Religion in order to get a clearer idea of the thinking of this great reformer.  It helps that this year is the 500th anniversary of his birth.  Unfortunately, Calvin (and Calvinism in general) get a lot of unfair treatment by others simply because people do not try to understand Calvin.  Here are a couple myths that I have discovered:
Calvin ruled Geneva with an iron fist.
Wrong. He wasn't a citizen until late in his life. He didn't vote. He was fired after only two years and sent packing unceremoniously. Do dictators get fired? To be sure, especially after he returned from exile his influence did grow considerably and he was probably the leading personality in the city by the 1550s. Nevertheless, the small city council (petit conseil) over ruled Calvin on at least two key issues. He wanted to observe the Lord's Supper weekly and he wanted to include an absolution or a declaration of pardon in the liturgy. Both of these should have been minor issues for a tyrant but he was unable to persuade the people and the civil leadership to let him do it. These failures to persuade suggest that Calvin did not have the sort of absolute authority that many people imagine. Indeed, at the end of his life, Calvin himself worried that not only was the Genevan Reformation a failure but that the entire episode might be coming to nothing.
Calvin murdered Miguel Servetus.
Wrong. To be sure Calvin did "rat out" but only after the former showed up in Geneva, despite Calvin's warning of what the civil authorities would do to him and then Servetus appeared in church.
It was the 16th-century. All the magisterial Reformers agreed that the magistrate should not tolerate public heresy against the catholic faith. It was a crime against the civil code in most European cities and Genva was no exception. If the Protestant cities had not treated heresy as a civil crime, the Roman authories would have accused them of giving hospitality to heretics. They were condemned if they did and condemned if they didn't.
There's little doubt that Calvin thought that the civil magistrate should be able to put heretics to death, but the fact is that Calvin didn't put to death anyone, let alone Servetus. Further, judging by the period, Calvin was a piker. Two or three anti-Trinitarians were put to death in Heidelberg in 1570, over the objections of the magistrate, at the insistence of the ministers! Why all the outrage over Calvin and his one heretic and virtual silence over the exponentially greater crime in Heidelberg? Calvin's opponents then and now use the episonde as a way of shaming Calvin (and Calvinists). It's not really about history. It's about the politics of perception.
Posted by mark wolter at 11:14 AM No comments: Links to this post
Piper on the Economic Downturn
Watch this presentation on Preserving, Sustaining Grace by Dr. R.C. Sproul.
Isn't it great to know that our hope is not in our ability to stay faithful, but in Christ's power by the Holy Spirit working in us.
Life Together for 33 years
Read my twin brother's comments on being a twin.
Update:  I'll add some of my own random fun memories:
1.  Cutting through the dirt hill on the way home and Doug getting his moon boots stuck in the mud!
2.  Seeing my friend Ryan go over the largest bike jump known to man and seeing him do a cartwheel in the process!
3.  "Going in together" to pick wilting wild flowers for a cute girl after 4th grade.
4.  Dirt clod fights.
5.  Wiping out while sledding on the hill and faking an injury only to really get hit and get my face all scraped up!
6.  Doug was the backstroke phenom.
7.  Do you still remember what I did to Gary's hat?
8.  Backyard tackle snow football.  Even got 29 year old Gary to play with us once!
9.  Doug's Buffalo Bills #88 shirt and my Earl Campbell jersey.  We were cool!  Also the "I'm a Pepper" shirts were pretty smooth.
10.  "Those red-wing blackbirds can kill you!"  We believed it too.
Posted by mark wolter at 10:49 AM 3 comments: Links to this post
"Jesus Christ".  It was 1997 and my friend had suddenly spoken up in his college class with these two words, not in a cursing tone but in a reasoning one.  It was almost as if a gun had gone off.  You could hear a pencil drop.  Everyone knew that those two words were weighty, that they divide, and that whatever words would follow would carry a deep and powerful message for good or ill.
A couple weeks ago I was contemplating whether or not I should keep the words, "Jesus Christ" at the top of my blog.  I decided that I do not care what other people think.  I love Jesus Christ more than anything or anyone.  (Yes, even more than my precious wife and kids.)
These words by John Piper also encouraged me to stay the course:
..here at the beginning of 2009, I [say], “I love Jesus Christ.” And as I say it, I want to make clear what I mean:
I enjoy his ways and his words more than I enjoy the ways and words of anyone else.
I want his approval more than I want the approval of anyone else.
I feel more grateful to him for what he has done for me than I do to anyone else.
Posted by mark wolter at 1:39 PM No comments: Links to this post
Posted by mark wolter at 10:27 PM 2 comments: Links to this post
Son of Hamas Leader Converts to Christianity
This guy has guts for taking the way of peace. Please pray for him!
Posted by mark wolter at 10:01 PM No comments: Links to this post
Charlie Hall is fast becoming one of my favorite singer/musicians.  His lyrics and his heart behind them are just quality.  It all shows of a heart focused on and satisfied in God alone.  Enjoy!
Posted by mark wolter at 5:39 PM No comments: Links to this post
New Year Bible Reading Plans
If you are like me, one way you want to grow during this new year is by being a better student of God's Word.  Here are some great suggestions and plans to get your heart and mind fixed more on God and His Word this year.  Enjoy!


"We are pleased to scale our business in the Middle East and for the first time, make human-eye resolution virtual and mixed reality available for customers in the United Arab Emirates," said  Seppo Aaltonen, Chief Commercial Officer of Varjo. "Current global circumstances have accelerated the need for high-fidelity immersive tools. Together with our local resellers, Varjo can now support advanced virtual, augmented, and mixed reality use cases in the UAE by offering best-in-class, yet cost-effective solutions. These are the perfect tools to help drive the UAE's technology transformation over the next 50 years."


Anheuser-Busch's new CEO said the company's Belgium-based parent AB InBev is "very bullish" and committed to investing in the U.S. following the passage of President Trump's tax overhaul legislation.
"We just finished our board meeting two weeks ago, where we said that we are committed to the U.S. market and that we are still very bullish on the U.S." nt told FOX Business.
"We are also very excited because our plants that we have in the U.S, we are going to put in around $2 billion [worth] of investments," he said. An annoucement that the company made back in May, a few months into Trump's first term.
Eric Shephard, executive editor of Beer Insights, said that dip isn't just coming from millennials, but customers overall, who are opting for lower-calorie and lighter beers.
Bud Light in particular topped this year's best seller list, with over $5.65 billion worth of sales, according to a report from Beer Marketer's Insights. Budweiser, however, fell to the fourth spot, after its sister brand officially dethroned it from the top spot in 2001.
Doukeris said when talking about Bud sales, it's very hard for people to separate the numbers with the role the beverage has played in American history.
While Trump's tax overhaul won't directly impact the beverage giant's books since it's based internationally, Doukeris believes it will have an indirect effect.
"Personally, when we look at our business, we are more optimistic about what tax reform will do, because if our customers are doing well financially, this will come back to the overall economy and it will hopefully impact our business," he said.


clicking on the POP email accounts link and opening the email account from there.
button next to the no longer required email address.
Setting your default email.
To set your default email address:
3. Click on the Submit button. Your new default email address has now been set.


Families can talk about jazz culture shown in . Where did jazz come from?What made it such a powerful force in its heyday?
Why was bebop considered such a radical innovation when it was popularized in the 1940s?What made it different from other popular music of the period?


The Portland, Maine chorus ChoralArt seeks new Christmas ca019–2020 holiday season. Entries must be a cappella or keyboard ac of an English text, with a duration of about three minutes. More information, guidelines, and an application form can be found at www.choralart.org/carolcontest.


Chief Of Staff Mark Meadows Ends Compliance With January 6th Committee
Thompson appeared on MSNBC last week and told host Rachel Maddow that if a person claims they have not done anything wrong but asserts Fifth Amendment rights, it “says that you have something to hide.”
As a result, the only two Republican members of the committee, Rep. Pelosi, appointed Liz Cheney (R-WY) and Adam Kinzinger (R-IL). Both Cheney and Kinzinger are strident never-Trump Republicans.


Darcy Bergen founded Bergen Financial in 2003. He founded our firm based on a simple philosophy; to teach our clients how to employ strategies to help protect them from incurring losses on their savings. Losing money in the stock market is not the way to save or live in retirement. We aim to provide education, advice, and consulting to retirees and those planning for retirement.
Bergen Financial Gnnuity-based products. Our team of advisors has over 100 years of experience assisting clients. There is no need to guess. We will set up a plan and help you implement a strategy.
ents fully understand. Since we are an independent firm, we can present several different strategies, not a one-size-fits all. Wey.


Sports Physicals Encouraged as Student Athletes Go Back to School - Archived
London, Ky. (August 15, 2016) – Students arer.
Aexam (PPE), is similar to the doctor’s exam that most children experience before heading back to class, but it also n,” said said Jay Shah, MD, orthopedic surgeon, KentuckyOne Health Orthopedic Associates”
Students involved in athletics are encouraged toThis allows the physician time to address past or lingering injuries before the season starts.
Whthe same. During a p.
Each year, more than 2.6  Hke, can also be dangerous for young athletes
KentuckyOne Health Sports Medicine, part of KentuckyOne Health, offers a comprehensive range of services for athleteThe group offers programs focused at preventing injuries before they happen. This includes a knee injury prevention program, shoulder and elbow injury prevention program, dry needling to improve pain control and reduce muscle tension, and concussion management. It also includes neuropsychology sports screenings for athletes who have experienced a traumatic brain injuryaid Dr. Shah”


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For example the back streets and outer suburbs of Taipei contain hundreds of small independently owned factories, which produce components for more prominent companies. Using the lure of Western material goods, Xavier was successful in converting many Japanese citizens to Christianity..
In.".
And so, globalisation is a powerful process with both benefits and disadvantages. The economic future of Taiwan appears positive, however one may questions oneself in what issues will this throw up in the realms of social, cultural and environmental factors?
have created an impact all over the world Interview for thesis ap world history comparison essay prompts unforgettable experience essay spm informative essay on global warming assignment patent. Essay parents and teenagers dissertation in finance project creative writing narrative formateducation grad school admissions essay.Download </s> In some case, you will like these farmhouse bathroom sink.zz kitchen trends study inc, Timing last kitchen more than years double bowl somewhat popular single farmhouse sink type material stainless steel porcelain tripleThere are three important steps on the best way to design a bedroom onlin forth. Once you complete these necessities, you will have an thought of how a lotpossibly can combine and match which paint colours to make use of. You can even check to see the shadebunch of coloration combinationsr combinations you will use.
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Distraction is one of the top complaints of meditators -- and of pretty much every human being, in this era that has been dubbed the “info blitzkrieg.” In this episode, we’ll hear from Dr. Amishi Jha, who has spent years studying the impact of meditation on people who work in high stress professions and has collaborated with the military, first responders, and elite athletes. Her new book is about how to, in her words, focus without all the struggle, take back your attention from the pull of distraction, and function at your peak.Dr.,and author of a new bIn this episode, Dr. Jha talks about: peak mind; the neuroscience of attention; how and why meditation works for high stress groups; multitasking vs. task switching; simulation mode vs. mindful mode; and answers the burning question - what is the least amount of meditation minutes one can do and still derive all the advertised benefits?As Dr. Jha mentioned in the episode, she recorded a meditation in the Ten Percent Happier app to help you practice paying attention to your attention. Check it out by downloading the Ten Percent Happier app wherever you get your apps, tapping on the Singles tab, and searching for her meditation called "Find Your Flashlight.” Or, click here to play the meditation.And be sure to check out our new podcast, Twenty Percent Happier, available exclusively in the Ten Percent Happier app.amishi-jha-388aking a bigger perspective and staying grounded in the body helps us experience life’s inevitable stress without being taken over by it.About Alexis Santos:Alexis has practiced and taught Insight Meditation in both the East and Westa (a well respected meditation teacher in Burma whose teachings have attracted a global audience), and y -- a style of practice that's particularly useful during our crazy livese.To “Stepping Away from Stress,6ab4db4d-eeb9-4f7e-baca-14938ce9d57we’re going to do some mindful eavesdropping. You’re going to get a chance to listen in on a process that rarely, if ever, gets aired publicly. You’re going to hear real students talking to a real meditation teacher about real life issues -- issues in their meditation practice, and issues related to applying meditation to your everyday life.The meditation teacher for this episode is Matthew Hepburn. Matthew has spent the last decade teaching meditation in schools, prisons, and meditation centers around the country. He’s an incredibly skilled, wise, and funny teacher. He’s also worked for many years at Ten Percent Happier, where Matthew is a stalwart on the content team. We cover some fascinating issues, including: how meditation can make you braver at work; undercover practices you can do with your spouse or partner; how to find meaning in everyday annoyances; how to handle fear; and the one thing that will break any meditation practice. You can listen to 20% Happier exclusively on the Ten Percent Happier app and download today: https://10percenthappier.app.link/instaltthew-hepburn-38ew of us relish chaos and disruption, but they are facts of life, given the nonnegotiable nature of change. In this episode with Zenju Earthlyn Manuel, we’re going to talk about how to tune into the value of disruption, and learn how to sit with the chaos. Zenju Earthlyn Manuel is an ordained Zen priest, holds a Ph.D., r and development director for non-profit organizations. She is also a prolific author.In this conversation we’ll explore: what to do with the unknown and not having any answers; the power of a “sip of silence” (her term); what she means by the phrase “death as a doorway to tenderness;” how she defines tenderness - a word that can easily get bogged down in sloppy sentimentality; and what she meant when she wrote “I'm not advocating love as an answer to all of the ills of the world. Then again, it is just that simple to be love.”Content Warning: There are brief mentions of assault; spiritual, sexual, and substance abuse; and racism, including a recent incident Zenju experienced hersession walks you through a guided visualization imagining immersing yourself in a pond of loving-kindness and letting the water infuse you.About Diana Winston:where she also teaches mindfulness practices to the general public.Her easy-on-the-ears West Coast style rests on top of a rigorous scientific mind, and a vast amount of teaching experience. She has developed curriculum and taught mindfulness since the early 90’. She has taught mindful awareness to health professionals, leaders, teachers, activists, seniors, and adolescents in the US and Asia. A published researcher and author, Diana has also written for numerous meditation publications, where her daughter, Mira, often makes an appearance in her examples of bringing mindfulness to daily lifLoving-Kindness Visualizatio9d970c28-16b8-45df-82de-24d61bde4075talk a lot on this show about social connection, but in this episode we’re going to get super granular on how to actually do relationships better.Carole Robin and David Bradfordmbined total of 75 years. theyWe dive into the six hallmarks of what they call “exceptional relationships,” how to be honest and vulnerable without overdoing it, why the questions “how am I feeling?” and “how are you feeling?” are central to improving our communication, the inevitability of risk when you set out to deepen a relationship, and why meditation is helpful in all of this.arole-robin-david-bradford-385mental health community, there’s a new term: “Eco-Anxiety.” Our guest in this episode, Jay Michaelson, has been thinking hard about climate change for many, many years. Michaelson is a meditation teacher, rabbi, lawyer, activist, and journalist. And he is also a core teacher in the Ten Percent Happier app. He’s covered climate change extensively, and has taught environmental ethics at Boston University Law School and Chicago Theological Seminary. He has also been a leading environmental activist in religious communities. In this conversation, we talk about what Jay thinks some meditation teachers get wrong about climate change, what he calls the “delusion” that individual habit change can make an impact, how we can use meditation to engage more effectively in the kind of politics he says we need to move the needle on a systemic level, and how to use meditation and deep breathing to handle eco-anxieLearn how to develop joy in this guided session from Sebene.About Sebene Selassie:Joy8ab8948a-d112-423a-bf8b-78f1e6e2b29e’re talking about increasing happiness by connecting to nature. Guest Andreas Weber is a renowned philosopher, biologist, and writer based in Berlin. He is the author of many books, including Matter & Desire: An Erotic Ecology. He has a fascinating and surprising approach: calling for an “erotic” relationship to nature. Weber calls it “erotic ecology” and argues that we have been socialized to have an instrumental view of nature and instead wants us to be in a love relationship with nature. In this conversation, we talk about how to actually practice erotic ecology, what Weber means when he says love is the foundational principle of reality, how and why to make ourselves “edible,” and how Weber manages his own pessimism when it comes to climate chree your creativity with this light meditation and see where the momentum takes" e Art of Creative Freedom0216495-f77b-491e-b1c5-ad1de8eda6dc.Here are the steps for sending us a question for our upcoming Work Life Series:1. Go to a quiet place and open the default voice memo recording app on your phone.2. Hold the phone about 8-10 inches from your face, then tap “record.”3.r question is. Try to keep it to about a minute or so.4. Stop the recording, then check it to make sure it sounds clear.5. Email it to us at |||EMAIL_ADDRESS||| by September 27,one of my favorite talks so far on this podcast. How to be able to apply mindfulness to our daily lives. Thanks Dan for sharing with us all. Keep up the great work.
I love this! Can't wait for the book.
thank you for putting more Buddhist talks :) 10% happier is one of my favorite podcasts. just btw.
This episode was such a delight!
Great conversation. We are listening to the Seeing White series at work and it is so informative and helpful! One hopefully constructive critique: I hope in the near future there can be (good faith, constructive) dialogue between people such as Kendi and Biewen and those within the "heterodoxy" on the topic of anti-racism such as McWhorter. Biewen's remarks about those folks was a bit dismissive, and also not very informed.
Can't really get deep when he talkes all the time.


It sounds pretty obvious, but it's not a good idea to shoot fireworks toward homes.er and little yard space, you might want to avoid shooting them off at your house. Some cities and states actually have laws against fireworks. When lighting fireworks, be sure to do so in a spot with plenty of open space. It's very difficult to control where a firework goes after it's been lit. You want to avoid fireworks, or firework debris, from landing on your roof (or someone else's) altogether.
2. Clear Your Roof of Debris.
It's much easier for a firework to start a fire on your roof if there's something flammable up there. Before shooting off any fireworks, be sure to clear your roof of any debris. Dry leaves, sticks and other debris tend to gather in roof valleys and gutters. These type of materials can easily turn a small spark into a blazing fire. By clearing your roof prior to the fireworks, you'll decrease the risk of a house fire if a firework does accidentally land on your roof.
3. Trim Back Overhanging Trees.
Trees that are close to your home can also catch on fire if hit with fireworks. If a tree branch that's hanging over your roof catches fire, those flames can easily fall off and catch your roof on fire too. Avoid that situation altogether by trimming back any trees prior to shooting off fireworks, especially any dead limbs or branches. This is a great tip even if you're not shooting off fireworks, as summer storms can cause tree branches to fall on your home.
If you're concerned that fireworks, tree branches or anything else has compromised your roof this summer, give Above & Beyond Roofr staff is here to take care of all your roofing needs!


One of the most popular gemstones, Sapphire is a crystallizas rubys them.
00000Sapphires come in varying qualities from the dark, blackish blue usually sourced from Australia, to the beautiful corn- flower blue of the Kashmir stones. As well as differing qualities, Sapphire can be found in many colours ; pink, purple, yellow, lilac, green, white, and even orange.
00000Pink sapphire is a stone that is growing in popularity. More durable than pink tourmaline - it's closest colour rival - and with a brilliance that's hard to match, it has become a favourite with jewellery designers young and not quite so young. Like so much in the gemstone world iand accordingly prices have dropped to reflect this.
00000The sapphire is associated with wounds, and as an antidote for poisons. 00000It is believed to amplify intuitive ability, and is useful as an aid to meditation.
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Car insurance comparison site Gocompare.com has completed new research which suggests some 5 milion motorists could overpay for their car insurance, totalling over £1 billion.
Gocompare.com found that 19% of drivers only read and file their car insurance renewal quote and don’t compare it against that offered by the competition. This could result in them spendcar insurance.
CEO of Gocompare,com, Hayley Parsons, said ‘’
She went on to say ‘ow. The idea that one insurer will remain the most competitive from one year to the next or that customers will be rewarded for their loyalty couldn’t be further from the truth.’
According to the research switch to another car insurance company if they could save anything at all and over 80% said they would switch if they could save up to £75 a year.
By comparing their insurance renewal quote online, Gocompare.com show that their customers saved an average of £207 per year.
Gocompare.com was launched in 2006 to help people get the right insurance at the right price.
Today the comparison site compares home, motorbike, van and breakdown cover as well as car insurance.
Daniel Wilson says
Insurance companies prey on our apathy. In the current economic climate I would expect people are looking for ways to save money and this is one of the best places to start. New customers may also benefit from new customer discounts and the like.


and s.
This product is currently available in Watermelon and Lemon & Lime flavours.
It contains Caffeine and Creatine to help boost your performance during training and is also packed with a good serving of BCAAs to help with muscle repair and prevent further muscle breakdown.
We also loveThe final pre workout product on our list is Adapt Nutrition’s Pre Train.
Th.
Pre Train contains some of the best ingredients for a pre workout supplement, including Caffeine, Beta Alanine, Citrulline Malate, Taurine, Vitamin B6 and Arginine.
It’s currently available in both a Blue Raspberry and Apple flavour and mixes very well with water. It really tasted great.
We like this product a lot but once again it contains the artificial sweetener Sucralose.
So there you have it, our picks for the top pre workout supplements on the market today.
Our favourite product by far is 4 Gauge because it contains the best. It stands out as the top choice for a pre workout but you won’t go wrong with any of the four products selected abovet. 6 best pre workout foods
MORE: The latest tennis news </s> What do women want?
The midrise condo, to be launched this month near Bayview Ave. and Sheppard Ave. E., will offer a host of female-friendly features intended to appeal to women buyers while not alienating their male counterparts.
“A lot of the time a building is designed by a lot of men and too often there are not enough women in the room. This time I was almost like the token guy (Jade's architect, interior designer and sales team are female) and it was a very collaborative process.”
When the family-owned business decided to go ahead with the Jade condo project, it seemed only natural to apply what it already knows about female consumers.
“There are so many condo sites, and although we have a great location and a contemporary boutique building, we felt we needed to have a marketing edge and decided to approach the female angle,” explains Henry Strasser, a principal of Phantom Developments.
(Some features will be standard; others are upgrades.)
“The market has totally changed (since the Bay 1001 project 20 years ago),” says Strasser.
“It's very different how suites are marketed, with different launches. You'll have a broker launch, a VIP launch, one for outside agents, then the public. The process is different. I find the market is a lot more sophisticated now. The brochures are slicker. You have an Internet presence, and you have to be everywhere, in a lot of media.”
The female-friendly condo concept was embraced by designer Tania Richardson, a principal in Tomas Pearce Interior Design Consulting Inc. and her team.
She adds: “It's such a simple concept, yet no one has capitalized on it before. If there's a developer who understands women, it's the Strassers.”
Safety is a big concern for female buyers, so security features get high priority. There will be a 24-hour concierge, a well-lit underground parking garage and lots of mirrors in the hallways and common areas to offer visibility from all angles. As well, there are extra security cameras in the garage and elevators.
“We'll also have a fob-style access key, similar to what you get with a car, so it's easy to use when your hands are full,” says Richardson.
“There's nothing worse than that when you are trying to put on makeup,” says Richardson. “We've integrated face level lighting on either side of the mirror to help when women are applying makeup.”
The receptacles are in place and it's up to the buyer to install


Review of Medical Hypnosis. By Lewis R. Wolberg, M.D. (Volume 1: pp. 449; $5.50. Volume 2: pp. 513; $6.50.) New York: Grune and Stratton. 1948.
In the author's view the applications of hypnosis in treatment are wide and varied, but they should always be a part of a consistent scheme of psychotherapy. He discusses the use of hypnosis for the simple removal of a symptom, as a part of non-analytic types of psychotherapy, reassurance, and persuasion, and as a technique to aid progress in psycho-analysis. Nearly every chapter of the second volume is profusely illustrated with recorded conversations between doctor and patient which effectively display the author's methods. In a final note the use of hypnosis for public displays is deplored, and the author demands protective laws which would confine the use of hypnosis to the medical profession.


spins round which is triggered whenever the player finds three or more scatter symbols. You'll earn up to 2, coins for finding treasure chests, vases and ankhs, whilst it's up to 2, coins for finding mummified dogs, and up to 5, coins for sarcophaguses and Ra's all-seeing eGT is a gaming provider that has been with us quite some time. Upgrade and resend email.
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In 1995, Staz Software led by Chris Stasny, acquired the rights to market FutureBasic. Chris Stasny started this business with an upgraded version, namely FBII, and with his own development, a CASE tool namely the Program Generator (PG PRO) became available. existance of the Mac and Apple itself was in the news every week.
Major update releases introduced a full-featured Appearance Compliant runtime written by Robert Purves and the Carbon compliance of generated applications. Once completely carbonized to run natively on MacOS X, the FutureBasic IDE was called FB4 and first released in July 2004.
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Roots, what roots?
Keep the kids school projects if nothing else. My parents kept all my artwork and that's the only thing I'm happy to look back on (aside from pictures). Trust me, they will appreciate it and love it in the future and so will you. So, give away the clothes and recipes, but definitely don't throw out the school projects. Those are evidence of what your child can do 🙂 and they're very cute to see later on!
I second Kiki. Even though I make fun of it every year, Christmas wouldn't be Christmas without a certain felt gingerbread man…


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Data for different components of the survey are released in separate data files to facilitate a more focused analytic option for researchers and faster access over the Internet. Below is a list of the basic files in NHANES I Data Files, Documentation, Codebook 1971-75, as reviewed in the Data Structure and Contents module. The titles indicate the topic area contents.
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Attorney Willie Briscoe of his own firm, explained thatBidz shareholders and whether the board of d'' He added that ''at least one analyst at Yahoo!financial newshares is $1.25 per share.''
Briscoe said, ''Due toBidz stock, so we"


Buyer agrees(3days) of possession for a complete health examination. Once the puppy has been verified healthy the breeder assumes no further responsibility of the condition of the puppy since the care of the puppy is out of our control. If buyer fails to have this puppy examined within 72 hours of possession all parts pertaining to the future health of this puppy and this warranty are null and void between buyer and sellerd while in the care of the new owner” listing why this determination was made. This must be forwarded to the breeder immediately / on the same day of the exam. At this time return of the puppy will also be arranged. Puppy must be returned within 24 hours to the breeder. If the buyer does not decide to return the puppy, they will then take full responsibility of the future health of the puppy and the breeder will not be held responsible.
In the event that this puppy develops a serious genetic defect i.e. heart, lungs, kidneys within one year of this puppies date of birth, the breeder will require a written report from a licensed veterinarian on company letterhead and any and all documented proof of the disorder. Once the major problem has been determined and return of this  and this puppy along with all AKC paperwork and aforementioned veterinarian results and records are returned to the seller, (return of puppy with a serious genetic disorder is not required for a replacement puppy). If a puppy is euthanized without prior knowledge to the breeder or a deceased puppy may require a necropsy from a state laboratory to properly evaluate the condition of the dogble. No cash refunds will be given, only a replacement puppy. All Vel alwaystheShipping of the replacement puppy will also be the responsibility of the buyer. Seller will provide a replacement puppy within 12 months of return of this puppy.
(1) All puppies are examined by a licensed veterinarian and will receive a health certificate prior to leaving the breeders house and going to its new home. Puppies will have age appropriate vaccines and worming’s and will be considered healthy at time of sale.
(2) Buyer is responsible to continue proper care for this puppy including but not limited to proper feeding and water, continuing puppy vaccinations, additional worming’s and heartworm treatment on this puppy. If buyer fails to do so all health agreements in this warranty on this puppy are null and void.
(3)your puppy is determined unfit for sale by a licensed veterinarian prior to leaving breeders property.
(5) Breeder is not responsible for any vet costs, test, treatments, nor for any transportation cost receiving or returning this puppy, stress or any other related expenses.
(6) Shipment of this puppy by air carrier is at the risk of the buyer. All precautions will be taken to assure that your puppy is properly crated and cared for, but the breeder is not responsible for any unforeseen occurrences on the part of the air carrier, air personnel or airport handlers.
(7) There shall be no refunds/exchanges for any reason provided this puppy is fit for sale. This includes but not limited to inability to train, allergies, family disputes, size, or conformation of dog.
(8) This warranty does not cover any neglect or injuries to this puppy that may have incurred once the buyer has taken ownership of this puppy.
(9) Breeder makes no guarantee that this puppy is show quality or breeding stock as this cannot be determined at such a young age.
(10) There is no guarantee on the adult size or color of this puppy as either can be determined by diet and possible shading of the color and is too young to determine.
(11) All my Chihuahuas are AKC registered. Buyer agrees to start their registered name with I LUV if they are sold with AKC rights.
(12) If buyer is ever no longer able to care for this puppy properly the breeder will always take it back. No refunds will be given. Breeder requests to be notified if a puppy is placed in a new home.
(13) Buyer is aware of consumers rights 828-29 Florida statues.
(14) This Chihuahua is sold with the understanding that it will live indoors and be a part of your family.
(15) If a replacement puppy is to be given to buyer due to a serious genetic defect the breeder has up to 12 months to provide buyer with a replacement puppy.
(16) The breeder reserves the right puppy is agreed upon by breeder.
Please remember that your new puppy is a baby and should be treated like one. The changing of homes and leaving his/her friends and family is upsetting to the puppy and it needs to have time to adjust to its new home. He/she will take frequent naps until he/she is acclimated to its new home. It is my hope that this puppy will be a happy addition to your family and it will be loved and cared fort.
By accepting the above described puppy limited health guarantee.


Stylish aparthotel in the heart of Cardiff.
It’s more than just a hotel, choose from a collection of boutique one and two bedroom apartments to Urban studios – perfect for those visiting the city for a shorter stay. The development boasts social spaces, co-working and meeting spaces enabling you to live, work and meet all under one roof.


Kaanchi – The Unbreakable movie review News
 full of hope. I had put Subhash Ghai’s disasters Kisna and Yuvvraj behind me to watch Kaanchi, free from preconceived notions. The sight of Click here to read more
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SIndrani Chakraborty, it is believed that Ghai rechristened this newbie for for a grand entry in Bollywood. And when Kaanchi – The Unbreakable director was quizzed about his fetish for renaming his heroines, this is what he said,<<