On January 23, 2018, the telecommunications industry gathered for the inaugural Pacific Telecommunications Council (PTC) Innovation Awards ceremony, a special gala at PTC'18 in Honolulu,ne award that drew a lot of attention this year was "Best Networking Innovation," which went to PacketFabric, a NantWorks company, for its on-demand transatlantic Connectivity-as-a-Service (CaaS) collaboration with Aqua Comms DAC ("Aqua Comms"). PacketFabric also took home "Best Application/ Service Innovation" for its CaaS solution.


oilet Paper Free Expeditions
Man, I love mike’s illustrations.
Sphagnum, hobblebush and striped maple are among the toilet-trees favored in eastern US forests, but not along the Appalachian Trail please, unless the consensus suggests 360 degree views from the privy.
Not to mention as diaper material.
@slowhikeLocale: South East U.S.
 ‘pick’″!
William WrightMember
@farstar
Nick ShepherdMember
One thing that may differ from the 'wipe all you troubles away' paradigm is to use a dab of vaseline applied pre-emptively. Helps exegis of yesterdays dinner without leaving a trace. A quick brush with some grass (not always necessary if you've had enough fibre) and you're ready to hit the trail.
Cuts down on chafing too.
It basically uses a water bottle and one's hand and then thorough washing and disinfecting. Becoming more and more popular from what I hear. I still need to try it out myself.
River rocks are a cool idea. There are plenty of those everywhere. But pick them up along the trail? That seems to be against the entire idea of UL backpacking. After just a small handful of rocks, you will have added a couple of pounds, if not more, to you pants.


Although many computational methods have been developed to predict protein subcellular localization, most of the methods are limited to the prediction of single-location proteins. Multi-location proteins are either not considered or assumed not existing. However, proteins with multiple locations are particularly interesting because they may have special biological functions, which are essential to both basic research and drug discovery.
This paper proposes an efficient multi-label predictor, namely mGOASVM, for predicting the subcellular localization of multi-location proteins. Given a protein, the accession numbers of its homologs are obtained via BLAST search. Then, the original accession number and the homologous accession numbers of the protein are used as keys to search against the Gene Ontology (GO) annotation database to obtain a set of GO terms. Given a set of training proteins, a set of T relevant GO terms is obtained by finding all of the GO terms in the GO annotation database that are relevant to the training proteins. These relevant GO terms then form the basis of a T-dimensional Euclidean space on which the GO vectors lie. A support vector machine (SVM) classifier with a new decision scheme is proposed to classify the multi-label GO vectors. The mGOASVM predictor has the following advantages: (1) it uses the frequency of occurrences of GO terms for feature representation; (2) it selects the relevant GO subspace which can substantially speed up the prediction without compromising performance; and (3) it adopts an efficient multi-label SVM classifier which significantly outperforms other predictors. Briefly, on two recently published virus and plant datasets, mGOASVM achieves an actual accuracy of 88.9% and 87.4%, respectively, which are significantly higher than those achieved by the state-of-the-art predictors such as iLoc-Virus (74.8%) and iLoc-Plant (68.1%).
mGOASVM can efficiently predict the subcellular locations of multi-label proteins. The mGOASVM predictor is available online at<URL>
Support Vector MachineGene OntologySubcellular LocationLocative AccuracyQuery Protein
Knowing where a protein resides in a cell can help biologists elucidate the functions of the protein. With the accomplishment of the various large-scale genome sequencing projects, an exponentially growing number of new protein sequences have been discovered[1, 2]. Computation methods are required to automatically and accurately identify the subcellular locations of these proteins.
Conventional methods for subcellular-localization prediction can be roughly divided into sequence-based methods and annotation-based methods. Sequence-based methods include: (1) sorting-signals based methods, such as PSORT[3], WoLF PSORT[4], TargetP[5] and SignalP[6, 7]; (2) composition-based methods, such as amino-acid compositions (AA)[8], amino-acid pair compositions (PairAA)[8], gapped amino-acid pair compositions (GapAA)[9, 10], and pseudo amino-acid composition (PseAA)[11, 12]; and (3) homology-based methods, such as Proteome Analyst[13], PairProSVM[14] and other predictors[15–17].
Annotation-based methods make use of the correlation between the annotations (usually the functional annotations) of a protein and its subcellular localization. Among them, methods based on Gene Ontology (GO) information are more attractive. Gene Ontology (GO)a is a set of standardized vocabularies that annotate the function of genes and gene products across different species. The term ‘ontology’ originally refers to a systematic account of existence. In the GO database, the annotations of gene products are organized in three related ontologies: cellular components, biological processes, and molecular functions. Cellular components refer to the substances that constitute cells and living organisms. Example substances are proteins, nucleic acids, membranes, and organelles. Majority of these substances are located within the cells, but there are also substances locating outside the cells (extracellular areas). A biological process is a sequence of events achieved by one or more ordered assemblies of molecular functions. A molecular function is achieved by activities that can be performed by individual or by assembled complexes of gene products at the molecular level.
As a result of the GO Consortium annotation effort, the Gene Ontology Annotation (GOA) databaseb has become a large and comprehensive resource for proteomics research[18]. The database provides structured annotations to non-redundant proteins from many species in UniProt Knowledgebase (UniProtKB)[19] using standardized GO vocabularies through a combination of electronic and manual techniques. The large-scale assignment of GO terms to UniProtKB entries (or accession numbers) was done by converting a proportion of the existing knowledge held within the UniProKB database into GO terms[18]. The GO annotation database also includes a series of cross-references to other databases. Thus, the systematic integration of GO annotations and UniProtKB database can be exploited for subcellular localization. Specifically, given the accession number of a protein, a set of GO terms can be retrieved from the GO annotation database filec. In UniProKB, each protein has an accession number, and in the GO annotation database, each accession number may be associated with zero, one or more distinct GO terms. Conversely, one GO term may be associated with zero, one, or many different accession numbers. This means that the mappings between accession numbers and GO terms are many-to-many.
From the perspective of GO terms extraction, the GO-based predictors can be classified into three categories: (1) using InterProScan[20] to search against a set of protein signature databases[21–25]; (2) using the accession numbers of proteins to search against the GO annotation database such as Euk-OET-PLoc[26], Hum-PLoc[27], Euk-mPLoc[28], Gneg-PLoc[29] and an integrated method[30]; and (3) using the accession numbers of homologous proteins retrieved from BLAST[31] to search against the GO annotation database, such as ProLoc-GO[32], iLoc-Virus[33], iLoc-Gneg[34] and Cell-PLoc 2.0[35].
However, there exist multi-location proteins that can simultaneously reside at, or move between, two or more different subcellular locations. Unfortunately, most of the existing methods are limited to the prediction of single-location proteins. These methods generally exclude the multi-label proteins or are based on the assumption that multi-location proteins do not exist. Actually, proteins with multiple locations play important roles in some metabolic processes that take place in more than one compartment, such as fatty acid β-oxidation in the peroxisome and mitochondria, and antioxidant defense in the cytosol, mitochondria and peroxisome[36].
There are a few predictors[33, 37, 38] specifically designed for predicting viral proteins, generated by viruses in various cellular compartments of the host cell or virus-infected cells. Studying the subcellular localization of viral proteins enables us to obtain the information about their destructive tendencies and consequences[33, 37, 38]. It is also beneficial to the annotation of the functions of viral proteins and the design of antiviral drugs. To the best of our knowledge, there are two predictors, namely Virus-mPLoc[37] and iLoc-Virus[33], capable of predicting multi-label viral proteins. iLoc-Virus performs better than Virus-mPLoc because the former has a better formulation for reflecting GO information and has a better way to predict the number of subcellular location sites of a query protein[33]. Recently, a method called KNN-SVM ensemble classifier[39] is proposed to deal with multi-label proteins, including viral proteins. It was found that the performance of the KNN-SVM predictor is comparable to iLoc-Virus and is better than Virus-mPLoc.
Conventional methods specializing for plant proteins, such as TargetP[5] and Predotar[40], can only deal with single-label proteins. Plant-mPLoc[41] and iLoc-Plant[42] are state-of-the-art predictors that can deal with single-label and multi-label proteins of plants. iLoc-Plant performs better than Plant-mPLoc because of the similar improvement as in iLoc-Virus versus Virus-mPLoc.
This paper proposes an efficient multi-label predictor, namely mGOASVM, for multi-label protein subcellular localization prediction. Here, the prefix “m” stands for multiple, meaning that the predictor can deal with proteins with multiple subcellular locations. mGOASVM is different from other predictors in that (1) it adopts a new decision scheme for an SVM classifier so that it can effectively deal with datasets containing both single-label and multi-label proteins; (2) it selects a set of distinct, relevant GO terms to form a more informative GO subspace; (3) it constructs the GO vectors by using the frequency of occurrences of GO terms instead of using 1-0 values[23, 37, 41] for indicating the presence or absence of some predefined GO terms. The results on two benchmark datasets and a newly created dataset full of novel proteins demonstrate that these three properties enable mGOASVM to predict multi-location proteins and outperform the state-of-the-art predictors such as iLoc-Virus and iLoc-Plant.
Because the cellular component GO terms have already been annotated with cellular component categories, it seems that what we need is to create a lookup table using the cellular component GO terms as the keys and the component categories as the hashed values. Such a naive solution, however, is undesirable, as will be explained below.
Although the cellular component ontology is directly related to the subcellular localization, we cannot simply use its GO terms to determine the subcellular locations of proteins. The reason is that some proteins do not have cellular component GO terms. Even for proteins annotated with cellular-component GO terms, it is inappropriate to use these terms only to determine their subcellular localizations. The reason is that a protein could have multiple cellular-component GO terms that map to different subcellular localizations, which are highly likely to be inconsistent with the true subcellular locations of proteins. Another reason is that proteins with annotated subcellular localization in Swiss-Prot may still be marked as ‘Cellular Component Unknown’ in the GO database[26]. Because of this limitation, it is necessary to use the other two ontologies as well because they are also relevant (although not directly) to the subcellular localization of proteins.
To exemplify the above discussion, we created a lookup table (Table1) and developed a table-lookup procedure to predict the subcellular localization of the proteins in the virus dataset (see Table2(a)). Table1 has two types of GO terms: essential GO terms and child GO terms. As the name implies, the essential GO terms[32] are GO terms that are essential or critical for the subcellular localization prediction. In addition to the essential GO terms, their direct descendants (known as child terms) also possess direct localization information. The relationships between child terms and their parent terms include ‘is a’, ‘part of’ and ‘occurs in’[43]. The former two correspond to cellular component GO terms and the third one typically corresponds to biological process GO terms. As we are more interested in cellular component GO terms, the ‘occurs in’ relationship will not be considered. For ease of reference, we refer to both essential GO terms and their child terms as ‘explicit GO terms’.
Explicit GO terms for the virus dataset
Explicit GO terms
Child terms
GO:<DIGITS> (Part of), GO:0046798 (Part of),
GO:0046806 (Part of), GO:0019013 (Part of),
GO:0019029 (Is a), GO:0019030
GO:0044385 (Part of), GO:0044160 (Is a),
GO:0044162 (Is a), GO:0085037 (Is a),
GO:0044200
Host ER∗
GO:0033655
GO:0048046 (Is a), GO:0044421
Explicit GO terms include essential GO terms and their child terms. The definition of essential GO terms can be found in[32]. Here the relationship includes ‘is a’ and ‘part of’ only, because only cellular component GO terms are analyzed here. Relationship: the relationship between child terms and their parent essential GO terms;
No. of Terms: the total number of explicit GO terms in a particular class.
*:host endoplasmic reticulum.
Breakdown of the (a) virus protein dataset and (b) plant protein dataset
(a) Viral protein dataset
No. of locative proteins
Total number of locative proteins
Total number of actual proteins
(b) Plant protein dataset
Total number of locative proteins
Total number of actual proteins
For each class in Table1, the child terms were obtained by presenting the corresponding essential GO term to the QuickGO server[44]d.
Given a query sequence, we first obtain its ‘GO-term’ set from the GO annotation database. Then, if one (or more than one) of the terms in this set matches an essential GO term in Table1, the subcellular location set of this query protein is predicted to be the one (or the ones) corresponding to the matched GO term(s). For example, if the set of GO terms contains GO:0019028, then this query protein is predicted as ‘Viral capsid’; or if the set of GO terms contains both GO:0030430 and GO:0042025, then this query protein is predicted as ‘host cytoplasm’ and ‘host nucleus’. Further, if none of the terms in this set matches any essential GO terms but one (or more than one) of the terms in this set match(es) any child terms in Table1, then the query protein is predicted as belonging to the class(es) associated with the child GO term(s). For example, if no essential GO terms can be found in the set but GO:0019030 is found, then the query protein is predicted as ‘Viral capsid’; or if GO:0044155, GO:0044166 and GO:0033655 are found, then the query protein is predicted as ‘host cell membrane’, ‘host endoplasmic reticulum’ and ‘host cytoplasm’.
A major problem of this table lookup procedure is that the GO terms of a query protein may contain many essential GO terms and/or have child terms spanning across more classes than the number of true subcellular locations, causing over-prediction. For example, in the virus dataset, 69, 14 and 3 (out of 207) proteins have explicit GO terms that map to two, three and four locations, and 121 (out of 207) proteins have explicit GO terms that map to one location. By comparing with the true locations, there are totally 139 proteins whose explicit GO terms are consistent with their true locations, of which there are 107 single-label proteins, 30 two-label proteins and 2 three-label proteins. This means that only about 67% (139/207) proteins are likely to be predicted correctlyd. This analysis suggests that direct table lookup is not a desirable approach and this motivates us to develop machine learning methods for GO-based subcellular localization prediction.
In this paper, the virus dataset used in Virus-mPLoc[37] and iLoc-Virus[33] and the plant dataset used in Plant-mPLoc[41] and iLoc-Plant[42] were used to evaluate the performance of mGOASVM.
The virus dataset was created from Swiss-Prot 57.9. It contains 207 viral proteins distributed in 6 locations (see Table2(a)). Of the 207 viral proteins, 165 belong to one subcellular locations, 39 to two locations, 3 to three locations and none to four or more locations. This means that about 20% of proteins are located in more than one subcellular location. The sequence identity of this dataset was cut off at 25%.
The plant dataset was created from Swiss-Prot 55.3. It contains 978 plant proteins distributed in 12 locations (see Table2(b)). Of the 978 plant proteins, 904 belong to one subcellular locations, 71 to two locations, 3 to three locations and none to four or more locations. In other words, 8% of the plant proteins in this dataset are located in multiple locations. The sequence identity of this dataset was cut off at 25%.
To facilitate performance comparison, the concepts of locative proteins[33, 37] and actual proteins were introduced here. If a protein exists in two different subcellular locations, it will be counted as two locative proteins; if a protein coexists in three locations, then it will be counted as three locative proteins; and so forth. But no matter how many subcellular locations a protein simultaneously resides, it will be counted as only one actual protein. Mathematically, denote Nloc as the total number of locative proteins, Nactas the total number of actual proteins, M as the number of subcellular locations, nact(m) (m=1,…,M) as the number of actual proteins coexisting in m subcellular locations. Then, the Nact and Nloc can be expressed as:
N act = ∑ m = 1 M n act
N loc = ∑ m = 1 M m · n act
In the virus dataset, M=6; and in the plant dataset, M=12. Then, from Eq. 1 and Eq. 2, we obtain
N act v = 165 + 39 + 3 = 207
N loc v = 1 × 165 + 2 × 39 + 3 × 3 + ∑ m = 4 6 m × 0 = 252
N act p = 904 + 71 + 3 = 978
N loc p = 1 × 904 + 2 × 71 + 3 × 3 + ∑ m = 4 12 m × 0 = 1055
where the superscript v and p stand for the virus and plant datasets, respectively. Thus, for the virus dataset, 207 actual proteins correspond to 252 locative proteins; and for the plant dataset, 978 actual proteins correspond to 1055 locative proteins. The breakdown of these two benchmark datasets were shown in Table2(a) and Table2(b).
In statistical prediction, leave-one-out cross validation (LOOCV) is considered to be the most rigorious and bias-free method[45]. Hence, LOOCV was used to examine the performance of mGOASVM. In each fold of LOOCV, a protein of the dataset (suppose there are N proteins) was singled out as the test protein and the remaining (N−1) proteins were used as the training data. This procedure was repeated N times and in each fold a different protein was selected as the test protein. This ensures that every protein in the dataset will be tested. Here, ‘proteins’ refers to ‘actual proteins’ rather than ‘locative proteins’; otherwise the training set will contain identical proteins distributed across multiple classes, which violates the SVM learning requirement that positive-class training patterns must be different from the negative-class training patterns.
The locative accuracy[46] and actual accuracy were used to measure the performance of multi-label predictors. Specifically, denote ℒ ( p i ) and ℳ ( p i ) as the true label set and the predicted label set for the i-th protein p i (i=1,…,Nact), respectively. Then, the overall locative accuracy is:
Λ loc = 1 N loc ∑ i = 1 N act | ℳ ( p i ) ∩ ℒ ( p i ) |
where |·| means counting the number of elements in the set therein and ∩ represents the intersection of sets. And the overall actual accuracy is:
Λ act = 1 N act ∑ i = 1 N act Δ [ ℳ ( p i ) , ℒ ( p i ) ]
Δ [ ℳ ( p i ) , ℒ ( p i ) ] = 1 , if ℳ ( p i ) ≡ ℒ ( p i ) 0 , otherwise.
According to Eq. 7, a locative protein is considered to be correctly predicted if any of the predicted labels matches any labels in the true label set. On the other hand, Eq. 8 suggests that an actual protein is considered to be correctly predicted only if all of the predicted labels match those in the true label set exactly. For example, for a protein coexist in, say, three subcellular locations, if only two of the three are correctly predicted, or the predicted result contains a location not belonging to the three, the prediction is considered to be incorrect. In other words, when and only when all the subcellular locations of a query protein are exactly predicted without any overprediction or underprediction, can the prediction be considered as correct. Therefore, the actual accuracy is stricter than the locative accuracy.
Despite its strict criteria, the actual accuracy is regarded to be more objective than the locative accuracy. Locative accuracy is liable to give biased performance measure when the predictor tends to over-predict, i.e., giving large | ℳ ( p i ) | for many p i . In the extreme case, if we predict every protein in the virus dataset to have all of the 6 subcellular locations, according to Eq. 7, the locative accuracy is 100%. But obviously, the predictions are wrong and meaningless. On the contrary, if we use the actual accuracy as the performance measure in this extreme case, the actual accuracy will be 0%, which definitely conforms to what we expect.
Comparison with state-of-the-art predictors
Table3(a) compares the performance of mGOASVM against two state-of-the-art virus-specialized multi-label predictors on the virus dataset. Both Virus-mPLoc[37] and iLoc-Virus[33] use the accession numbers of homologs returned from BLAST[31] as searching keys to retrieve GO terms from the GOA database. The KNN-SVM ensemble classifier[39] uses the true accession number of proteins directly as input. For a fair comparison with these two predictors, the performance of mGOASVM shown in Table3(a) was obtained by using the accession numbers of homologous proteins as the searching keys. Like Virus-mPLoc and iLoc-Virus, mGOASVM uses BLAST[31] to find the homologs and then uses the accession numbers of the homologs as the searching keys. Here, mGOASVM selects the top homolog for each protein. If BLAST cannot find a homolog for a protein, we assign zeros to all entries of the corresponding GO vectors. In the virus dataset, a homolog can always be found for every protein.
Comparing mGOASVM with state-of-the-art multi-label predictors based on leave-one-out cross validation (LOOCV) using (a) the virus dataset and (b) the plant dataset
(a) Performance on the viral protein dataset
LOOCV locative accuracy
Virus-mPLoc [[37]]
KNN-SVM [[39]]
iLoc-Virus [[33]]
8/8 = 100.0%
19/33 = 57.6%
Host ER
9/20 = 45.0%
20/20 = 100.0%
Overall Locative Accuracy
152/252 = 60.3%
Overall Actual Accuracy
155/207 =74.8%
(b) Performance on the plant protein dataset
Plant-mPLoc [[41]]
iLoc-Plant [[42]]
72/182 = 39.6%
2/22 = 9.1%
150/150 = 100.0%
672/1055 = 63.7%
1015/1055 =96.2%
“–” means the corresponding references do not provide the overall actual accuracy. KNN-SVM: the KNN-SVM ensemble classifier proposed in[39]. Host ER: Host endoplasmic reticulum.
Table3(b) compares the performance of mGOASVM against two state-of-the-art plant-specialized multi-label predictors on the plant dataset. Plant-mPLoc[41] uses similar methods as Virus-mPLoc, and iLoc-Plant[42] uses similar methods as iLoc-Virus. Here mGOASVM also selects the top homolog for each protein.
As shown in Table3, for the virus dataset, mGOASVM performs significantly better than Virus-mPLoc and iLoc-Virus; for the plant dataset, mGOASVM also performs remarkably better than Plant-mPLoc and iLoc-Plant. In the virus dataset, both the overall locative accuracy and overall actual accuracy of mGOASVM are more than 14% (absolute) higher than iLoc-Virus (96.8% vs 78.2% and 88.9% vs 74.8%, respectively); and in the plant dataset, the corresponding two measures are more than 19% (absolute) higher than iLoc-Plant (96.2% vs 71.7% and 87.4% vs 68.1%, respectively). mGOASVM also performs significantly better than KNN-SVM ensemble classifier in terms of overall locative accuracy (96.8% vs 80.7%); except for the host cytoplasm, mGOASVM is more accurate than KNN-SVM in predicting all subcellular locations. The results on both datasets demonstrate that mGOASVM is more capable of handling multi-label problems than Virus-mPLoc, iLoc-Virus, KNN-SVM ensemble classifier, Plant-mPLoc and iLoc-Plant. As for the individual locative accuracy, in the virus dataset, except for the “viral capsid” for which all of mGOASVM, Virus-mPLoc and iLoc-Virus reach 100%, the locative accuracies of mGOASVM are remarkably higher than those of Virus-mPLoc and iLoc-Virus; while in the plant dataset, the individual locative accuracies of mGOASVM for all of the 12 locations are impressively higher than those of Plant-mPLoc and iLoc-Plant.
Kernel selection and optimization
A support vector machine (SVM) can use linear, RBF or polynomial function as its kernel. Some works[9, 47] have demonstrated that RBF kernels achieve better results than linear and polynomial kernels. However, our results show that linear SVMs perform better in our case. Table4 shows the performance of mGOASVM using different types of kernel functions with different parameters based on leave-one-out cross validation (LOOCV) using the virus dataset. For RBF SVM, the kernel parameter σ was selected from the set {2−2,2−1,…,25}. For polynomial SVM, the degree of polynomial was set to either 2 or 3. The penalty parameter (C) was set to 0.1 for all cases. Table4 shows that SVMs that use the linear kernel perform better than that with RBF and polynomial kernels. This is plausible because the dimension of GO vectors is larger than the number of training vectors, aggravating the curse of dimensionality problem in non-linear SVMs[48]. The over-fitting problem becomes more severe when the degree of non-linearity is high (small σ), leading to degradation in performance, as demonstrated in Table4. In other words, highly nonlinear SVMs become vulnerable to overfitting due to the high-dimensionality of the GO vectors.
Performance of mGOASVM using different kernels with different parameters based on leave-one-out cross validation (LOOCV) using the virus dataset
Locative accuracy
Actual accuracy
σ=2−2
σ=21
5/207 = 2.4%
Polynomial SVM
The penalty parameter (C) was set to 0.1 for all cases. σ is the kernel parameter for the RBF SVM; d is the polynomial degree in the Polynomial SVM.
Analysis of mGOASVM
Table5 shows the performance of the GO-vector construction methods. Linear SVMs were used in both cases, and the penalty factor was set to 0.1. The results show that term-frequency (TF) achieves a bit better performance than 1-0 value in the locative accuracy, but performs almost 2% and 7% better than 1-0 value in the actual accuracy for the virus dataset and the plant dataset, respectively, which demonstrates that the frequencies of occurrences of GO terms could also provide information for subcellular locations. The results are biologically relevant because proteins of the same subcellular localization are expected to have a similar number of occurrences of the same GO term. In this regard, the 1-0 value approach is inferior because it quantizes the number of occurrences of a GO term to 0 or 1. Moreover, the more remarkable improvement achieved for the plant dataset than that for the virus dataset also suggests that the term-frequency (TF) construction method can boost the performance more impressively for datasets with larger size and more multi-label proteins.
Performance of different GO-vector construction methods based on leave-one-out cross validation (LOOCV) for (a) the virus dataset and (b) the plant dataset
GO Vector construction
1-0 value
Term-frequency
1014/1055 = 96.1%
To reveal that the high locative accuracies of mGOASVM are due to the capability of mGOASVM rather than due to over-prediction, we have investigated the distributions of the number of predicted labels in both virus and plant datasets. We consider | ℳ ( p i ) | and | ℒ ( p i ) | (i=1,…,Nact) in Eq. 7 as the number of predicted labels and the number of true labels for the i-th protein, respectively. The distributions of the number of labels predicted by mGOASVM are shown in Table6. Denote n k o , n k e or n k u as the number of proteins that are over-, equal-, and under-predicted by k (k=0,…,5 for the virus dataset and k=0,…,11 for the plant dataset) labels. Also denote N o , N e or N u as the total number of proteins that are over-, equal-, and under-predicted, respectively. Here, over-prediction, equal-prediction and under-prediction are respectively defined as the number of predicted labels that is larger than, equal to, and smaller than the number of true labels. Table6 shows that proteins that are over- or under-predicted account for a small percentage of the datasets only (8.7% and 1.0% over- and under-predicted in the virus dataset, 8.7% and 1.4% over- and under-predicted in the plant dataset). Even among the proteins that are over-predicted, most of them are over-predicted by one location only. These include all of the 18 proteins in the virus dataset, and 83 out of 85 in the plant dataset. None of the proteins in the virus dataset are over-predicted by more than 1 location. Only 2 out of 85 proteins in the plant dataset are over-predicted by 2 locations, and none are over-predicted by more than 2 locations. As for under-prediction, all of the under-predicted proteins are only under-predicted by 1 location in both datasets. These results demonstrate that the over-prediction and under-prediction percentages are small, which suggests that mGOASVM can effectively determine the number of subcellular locations of a query protein.
Distribution of the number of labels predicted by mGOASVM for proteins in the virus and plant datasets
n k o , n k e or n k u
(N o , N e or N u )/Nact
k >2
| ℳ ( p i ) | > | ℒ ( p i ) |
18/207 = 8.7%
| ℳ ( p i ) | = | ℒ ( p i ) |
Equal-prediction
| ℳ ( p i ) | < | ℒ ( p i ) |
Under-prediction
ℳ ( p i ) : Number of predicted labels for the i-th (i=1,…, Nact) protein; ℒ ( p i ) : Number of the true labels for the i-th protein; Over-prediction: the number of predicted labels is larger than that of the true labels; Equal-prediction: the number of predicted labels is equal to that of the true labels; Under-prediction: the number of predicted labels is smaller than that of the true labels; n k o , n k e or n k u : the number of proteins that are over-, equal-, or under-predicted by k (k=0,…,5 for the virus dataset and k= 0,…,11 for the plant dataset) labels, respectively; No, Ne or Nu: the total number of proteins that are over-, equal-, or under-predicted, respectively.
Table7 shows the performance of mGOASVM with different inputs and different numbers of homologous proteins for the virus and plant datasets. The input data can be of three possible types: (1) accession number only, (2) sequence only and (3) both accession number and sequence. mGOASVM can extract information from these inputs by producing multiple GO vectors for each protein. Denote #homo as the number of homologous proteins, where #homo∈{0,1,2,4,8} for the virus dataset and #homo∈{0,1,2} for the plant dataset. For different combinations of inputs and numbers of homologs, the number of distinct GO terms can be different. Typically, the number of distinct GO terms increases with the number of homologs.
Performance of mGOASVM with different inputs and different numbers of homologous proteins for (a) the virus dataset and (b) the plant dataset
N d
S + AC
S: Sequence; AC: Accession Number; # homo: Number of homologs used in the experiments; N d (GO): Number of Distinct GO Terms. # homo=0 means only the true accession number is used.
Table7 shows that the number of homologs can affect the performance of mGOASVM. The results are biologically relevant because the homologs can provide information about the subcellular locations. However, more homologs may bring redundant or even noisy information, which are detrimental to the prediction accuracy. For example, in the plant dataset, the performance of using one homolog is better than that of using two (87.4% vs 63.1%), which in turn suggests that we should limit the number of homologs to avoid bringing irrelevant information. Moreover, as can be seen from Table7, the performance achieved by mGOASVM using sequences with the top homolog are comparable to that of mGOASVM using the accession number only.
Table7 shows that mGOASVM using both sequences and accession numbers performs better than using sequences only, but worse than using accession numbers.
Table8 shows the performance of mGOASVM for multi-location proteins using different inputs. Denote l ( l = 1 , … , ℒ ) as the number of co-locations. As the maximum number of co-locations in both datasets is 3, the individual actual accuracies for l (l=1,…,3) are shown in Table8. Note that high actual accuracies for l>1 are more difficult to achieve than that for l=1, since not only the number of subcellular locations for a protein should be predicted correctly, but also the subcellular locations should be predicted precisely. As can be seen, mGOASVM achieves high performance not only for single-label proteins (the column corresponding to l=1), but also for multi-label proteins (the columns corresponding to l=2 and l=3). The results demonstrate that mGOASVM can tackle multi-label problems well.
Performance of mGOASVM on (a) the virus dataset and (b) the plant dataset
Actual accuracy of protein groups
3/3 = 100%
S: Sequence; AC: Accession Number; # homo: Number of homologs used in the experiments; l (l=1,…,3): Number of co-locations. # homo=0 means only the true accession number is used.
To further demonstrate the effectiveness of mGOASVM, a plant dataset containing novel proteins was constructed by using the criteria specified in[41, 42]. The complete procedures of constructing the novel dataset can be found in the mGOASVM web-server. Specifically, to ensure that the proteins are really novel to mGOASVM, the creation dates of these proteins should be significantly later than the training proteins (from the plant dataset) and also later than the GOA database. Because the plant dataset was created in 2008 and the GOA database used was released on 08-Mar-2011, we selected the proteins that were added to Swiss-Prot between 08-Mar-2011 and 18-Apr-2012. Moreover, proteins with multiple subcellular locations that falls within the 12 classes specified in Table2(b) were included. After limiting the sequence similarity to 25%, 175 plant proteins distributed in 12 subcellular locations (see Table9) were selected. Of the 175 plant proteins, 147 belong to one subcellular location, 27 belong to two locations, 1 belong to three locations and none to four or more locations. In other words, 16% of the plant proteins in this novel dataset are located in multiple locations. The protein sequences of this new dataset can be donwloaded from the mGOASVM server.
Breakdown of the new plant dataset
Total number of locative proteins
Total number of actual proteins
The dataset was constructed from Swiss-Prot created between 08-Mar-2011 and 18-Apr-2012. The sequence identity of the dataset is below 25%.
Because the novel proteins were recently added to Swiss-Prot, many of them have not been annotated in the GOA database. As a results, if we used the accession numbers of these proteins to search against the GOA database, the corresponding GO vectors will contain all zeros. This suggests that we should use the ACs of their homologs as the searching keys, i.e., the procedure shown in Figure1 using sequences as input should be adopted. However, we observed that for some novel proteins, even the top homologs do not have any GO terms annotated to them. To overcome this limitation, the following procedure was adopted. For the proteins whose top homologs do not have any GO terms in the GOA database, we used the second-top homolog to find the GO terms; similarly, for the proteins whose top and 2-nd homologs do not have any GO terms, the third-top homolog was used; and so on until all the query proteins can correspond to at least one GO term. In the case where BLAST fails to find any homologs, we used the method PseAA[11] as a back-up. In this dataset, among 175 proteins, 5 of them require to use the backup method.
Flowchart of mGOASVM for three cases: (1) using accession numbers only; (2) using sequences only; (3) using both accession numbers and sequences. AC: Accession Number; S: Sequence. Part II does not exist for Case 1, and Part I does not exist for Case 2. Case 3 requires using both Part I and Part II. The score fusion implements Eq. 12.
Because BLAST searches were used in the above procedure, the prediction performance will depend on the closeness (degree of homology) between the training proteins and test proteins. To determine the number of test proteins that are close homologs of the training proteins, we performed a BLAST search for each of the test proteins. The E-value threshold was set to 10 so that none of the proteins in the lists returned from BLAST have E-value larger than 10. Then, we identified the training proteins in the lists based on their accession numbers, and recorded their corresponding E-values.
Figure2 shows the distribution of the E-values, which quantify the closeness between the training proteins and test proteins. If we use a common criteria that homologous proteins should have E-value less than 10−4, then 74 out of 175 test proteins are homologs of training proteins, which account for 42% of the test set. Note that this homologous relationship does not mean that using BLAST’s homology transfers can predict all of the test proteins correctly. In fact, BLAST’s homology transfers (based on the CC field of the homologous proteins) can only achieve a prediction accuracy of 26.9% (47/175). As the prediction accuracy of mGOASVM on this test set (see Table10) is significantly higher than this percentage, the extra information available from the GOA database plays a very important role in the prediction.
Distribution of the closeness between the novel testing proteins and the training proteins. The closeness is defined as the BLAST E-values of the training proteins using the test proteins as the query proteins in the BLAST searches. Number of Proteins: The number of training proteins whose E-values fall into the interval specified under the bar. Small E-values suggest that the corresponding novel proteins are close homologs of the training proteins.
Comparing mGOASVM with a state-of-the-art multi-label plant predictor based on independent tests using the new plant dataset
Independent test locative accuracy
0/1 = 0%
Table10 shows the prediction performance of mGOASVM on this novel protein dataset. As explained earlier, to ensure that these proteins are novel to mGOASVM, 978 proteins of the plant dataset (See Table2(b)) were used for training the classifier. We compared mGOASVM with the Plant-mPLoc[41] web-servere. As shown in Table10, mGOASVM performs significantly better than Plant-mPLoc. The overall locative accuracy and the overall actual accuracy of mGOASVM are more than 17%, 21% higher than those of Plant-mPLoc, respectively (locative accuracy 60.3% vs 42.7%, and actual accuracy 55.4% vs 34.3%). For most of 12 individual subcellular locations, mGOASVM outperforms Plant-mPLoc, except in cell membrane and peroxisome. Given the novelty and multi-label properties of these proteins and the low sequence similarity (below 25%), the locative accuracy of 60.3% and the actual accuracy of 55.4% achieved by mGOASVM are fairly high. On the other hand, due to the scarcity of data, mGOASVM does not perform well in some subcellular locations, such as cell wall and plastid. But the situation will be improved when more and more proteins are available for training our SVM classifiers.
mGOASVM possesses several desirable properties that make it outperform Virus-mPLoc[37], iLoc-Virus[33], Plant-mPLoc[41] and iLoc-Plant[42], which are specified subsequently.
GO-Vector construction
Virus-mPLoc and Plant-mPLoc construct GO vectors by using 1-0 value to indicate the presence and absence of some predefined GO terms. This method is simple and logically plausible, but some information will be inevitably lost because it quantizes the frequency of occurrences of GO terms to either 1 or 0. The GO vectors in iLoc-Virus and iLoc-Plant contain more information than those in Virus-mPLoc and Plant-mPLoc, because the former two consider not only the GO terms of the query protein but also the GO terms of its homologs. Specifically, instead of using 1-0 value, each element of the GO vectors in the iLoc-Virus and iLoc-Plant represents the percentage of homologous proteins containing the corresponding GO term. However, the method ignores the fact that a GO term may be used to annotate the same protein multiple times under different entries in the GO annotation database. On the contrary, mGOASVM uses the frequency of occurrences of GO terms to construct the GO vectors. Intuitively, this is because proteins of the same subcellular localization tend to be annotated by similar sets of GO terms. The advantages of using the GO term-frequency count as features is evident by the superior results in Table5.
Capability of handling multi-label problems
An efficient way to handle multi-label problems is to predict the number of labels for each sample first, and then to predict the specific label set for each sample according to the order of the scores. Let us compare mGOASVM with two kinds of existing approaches.
When predicting the number of subcellular locations for a query protein, iLoc-Virus and iLoc-Plant determine the number of labels of a query protein based on the number of labels of its nearest training sample. mGOASVM, on the contrary, determines the number of labels for a query protein by looking at the number of positive-class decisions among all of the one-vs-rest SVM classifiers. Therefore, the number of labels depends on the whole training set as opposed to the query protein’s nearest neighbor in the training set.
As opposed to Virus-mPLoc and Plant-mPLoc which require a pre-defined threshold, our mGOASVM adopts a machine learning approach to solving the multi-label classification problem. The predicted class labels in mGOASVM are assigned based on the SVMs that produce positive responses to the query protein.
In summary, the superiority of mGOASVM in handling multi-label problems is evident in Table3.
From the machine learning perspective, prediction of multi-location proteins is a multi-label learning problem. Approaches to addressing this problem can be divided into types: problem transformation and algorithm adaptation[49]. The multi-label KNN classifiers used in iLoc-Plant and iLoc-Virus belong to the first type whereas our multi-label SVM classifier belongs to the second type. While our results show that multi-label SVMs perform better than multi-label KNN, further work needs to be done to compare these two types of approaches in the context of multi-label subcellular localization.
GO subspace selection
To facilitate the sophisticated machine learning approach for the multi-label problem, GO subspace selection is adopted. Unlike the traditional methods[33, 37, 41, 42] which use all of the GO terms in the GO annotation database to form the GO-vector space, mGOASVM selects a relevant GO subspace by finding a set of distinct, relevant GO terms. With the rapid growth of the GO database, the number of GO terms is also increasing. As of March 2011, the number of GO terms is 18656, which means that without feature selection, the GO vectors will have dimension 18656. This imposes computational burden on the classifier, especially when leave-one-out cross validation is used for evaluation. There is no doubt that many of the GO terms in the full space are redundant, irrelevant or even detrimental to prediction performance. By selecting a set of distinct GO terms to form a GO subspace, mGOASVM can reduce the irrelevant information and at the same time retain useful information. As can be seen from Table7, for the virus dataset, around 300 to 400 distinct GO terms are sufficient for good performance. Therefore, using GO subspace selection can tremendously speed up the prediction without compromising the performance.
This paper proposes an efficient multi-label predictor – mGOASVM – to predict the subcellular locations of multi-label proteins. By using the accession numbers of query proteins as the searching keys to search against the GO annotation database, the GO terms of each query protein are retrieved. For proteins without an accession number, BLAST is used to find their homologs and the accession numbers of the homologs are used as the searching keys. Then the GO terms are used to construct the GO vectors, which are subsequently recognized by support vectors machine (SVM) classifiers equipped with a decision strategy that can produce multiple class labels for a query protein.
Comparing with the exsiting predictors, mGOASVM has the following advantages: (1) the improved SVM classifier used in mGOASVM can effectively deal with multi-label problems; (2) it selects a relevant GO subspace from the full GO vector space by using a set of distinct GO terms; and (3) it constructs the GO vectors by using the frequency of occurrences of GO terms instead of using 1-0 values for indicating the presence or absence of some predefined GO terms.
Experimental results demonstrate that mGOASVM can efficiently predict the subcellular locations of multi-label proteins. This work also demonstrates that it is not necessary to use a large number of homologous accession numbers for searching the GO annotation database. In fact, our results strongly suggest that using the top homologous accession number is already sufficient. For readers’ convenience, mGOASVM is available online at<URL>
The mGOASVM predictor uses accession numbers, amino acid (AA) sequences, and combination of both as input. The prediction process is divided into three parts: (1) GO terms extraction, (2) GO-vector construction and (3) multi-label one-vs-rest SVM classification.
Retrieval of GO terms
Given a query protein, mGOASVM can handle three possible cases: (1) only the accession number is known, (2) only the amino acid sequence is known, and (3) both accession number and amino acid sequences are known. For proteins with known accession numbers, their respective GO terms are retrieved from the GOA database using the accession numbers as the searching keys. For a protein without an accession number, its amino acid sequence is presented to BLAST[31] to find n homologs, whose accession numbers are then used as keys to search against the GOA database. This results in n sets of GO terms, one set for each homologous accession number. For a protein with both accession number and amino acid sequence, the accession number and the accession numbers of its homologs are used as searching keys, resulting in (n + 1) sets of GO terms.
In this work, we considered different numbers of homologs (1, 2, 4, and 8 for the virus dataset, and 1 and 2 for the plant dataset) to investigate how the number of homologs affects the prediction performance.
Note that the gene association filef that we downloaded from the GOA database does not provide any subcellular localization labels. This file only allows us to create a hash table storing the association between the accession numbers and their corresponding GO terms. This hash table covers all of the accession numbers in the GOA database released on 08-Mar-2011, meaning that it will also cover the 207 accession numbers in the virus dataset (dated 22-Sept-2009) but not the accession numbers in the new plant dataset. It is important to emphasize that given a query protein, having a match in this hash table does not mean that a subcellular-localization assignment can be obtained. In fact, having a match only means that a non-null GO vector can be obtained. After that, the SVMs play an important role in classifying the non-null GO vector.
Construction of GO vectors
Given a dataset, we used the procedure described in the last subsection to retrieve the GO terms of all of its proteins. Then, we determined the number of distinct GO terms corresponding to the dataset. Suppose T distinct GO terms were found; these GO terms form a GO Euclidean space with T dimensions. For each protein in the dataset, we constructed a GO vector by matching its GO terms to all of the T GO terms. We have investigated two approaches to determine the elements of the GO vectors.
1-0 value. In this approach, each of the T GO terms represents one canonical basis of a Euclidean space, and a protein is represented by a point in this space with coordinates equal to either 0 or 1. Specifically, the GO vector of the i-th protein is denoted as:
p i = a i , 1 ⋮ a i , j ⋮ a i , T where a i , j = 1 , GO hit 0 , otherwise
where ‘GO hit’ means that the j-th GO term appears in the GO term search result using the accession number of the i-th protein or its homolog(s) as the searching key.
Term-Frequency (TF). This approach is similar to the 1-0 value approach in that a protein is represented by a point in the T-dim Euclidean space. However, unlike the 1-0 approach [37, 41] , it uses the number of occurrences of individual GO terms as the coordinates. Specifically, the GO vector p i of the i-th protein is defined as:
p i = b i , 1 ⋮ b i , j ⋮ b i , T where b i , j = f i , j , GO hit 0 , otherwise
where fi,j is the number of occurrences of the j-th GO term (term-frequency) in the i-th protein. The rationale is that the term-frequencies may also contain important information for classification and therefore should not be quantized to either 0 or 1. Note that bi,j’s are analogous to the term-frequencies commonly used in document retrieval.
Multi-label multi-class SVM classification
To predict the subcellular locations of datasets containing both single-label and multi-label proteins, a multi-label support vector machine (SVM) classifier is proposed in this paper. GO vectors are used for training the multi-label one-vs-rest SVMs. Specifically, for an M-class problem (here M is the number of subcellular locations), M independent binary SVMs are trained, one for each class. Denote the GO vector created by using the true accession number of the i-th query protein as qi,0and the GO vectors created by using the n homologous accession numbers as qi,j, j=1,…,n. Then, the score of the m-th SVM given the i-th query protein is
s m ( q i ) = ∑ j = 0 n w j ∑ r ∈ S m α m , r y m , r K ( p r , q i , j ) + b m
where S m is the set of support vector indexes corresponding to the m-th SVM, αm,rare the Lagrange multipliers, K(·,·) is a kernel function, and w j ’s are fusion weights such that ∑ j = 0 n w j = 1 . In this work, linear kernels were used, i.e., K(p r ,qi,j)=〈p r ,qi,j〉. ym,r∈{−1, + 1} are the class labels (here we call them “the transformed labels”), which are denoted as:
For single-label p r ,
y m , r = + 1 , if ℒ ( p r ) = m − 1 , otherwise.
For multi-label p r ,
y m , r = + 1 , if ℒ ( p r ) ⋂ { m } ≠ ∅ − 1 , otherwise.
where m∈{1,…,M}. Note that unlike the single-label problem where each protein has one and only one positive transformed label, a multi-label protein can have more than one positive transformed label.
Then the subcellular location(s) of the i-th query protein will be predicted as:
ℳ ∗ ( q i ) = ⋃ m = 1 M { m : s m ( q i ) > 0 } .
As can be seen, ℳ ∗ ( q i ) is a predicted set that may have zero, one, or more than one element, which enables us to make multi-label prediction. In case Eq. 15 does not produce a class label, i.e., ℳ ∗ ( q i ) = ∅ , the number of subcellular locations is set to one and the location is given by
ℳ ∗ ( q i ) = arg max m = 1 M s m ( q i ) .
Note that p r ’s in Eq. 12 represents the GO training vectors, which may include the GO vectors created by using the true accession numbers of the training proteins or their homologous accession numbers. We have the following three cases.
If only the true accession numbers are available, then only q i,0 ’s exist and p r ’s represent the GO training vectors created by using the true accession numbers only. In that case, q i,j(j=1,…,n) do not exist; w 0=1 and w j =0 (j=1,…,n). Then, Eq 12 can be written as:
s m ( q i ) = ∑ r ∈ S m α m , r y m , r K ( p r , q i , 0 ) + b m .
If only the amino acid sequences are known, then only the accession numbers of the homologous sequences can be used for training the SVM and for scoring. In that case, q i,0 does not exist and w 0=0; moreover, p r ’s represent the GO training vectors created by using the homologous accession numbers only.
If both accession numbers and amino acid sequences are known, then both true accession numbers and the accession numbers of the homologous sequences are used for training the SVM and for scoring. Then, q i,j(j=0,…,n) exist, and p r ’s represent the GO training vectors created by using both the true accession numbers and the homologous accession numbers.
In this work, 1, 2, 4 and 8 homologs were tried for the virus dataset, and 1 and 2 homologs were used for the plant dataset, respectively, i.e., n∈{1,2,4,8} and n∈{1,2} in Eq. 12, respectively. For convenience, equal weights for the true accession number and the accession numbers of homologs were adopted. Therefore, for Case 2, w0=0 and w j =1/n, j=1,…,n; and for Case 3, we set w j =1/(n + 1), j=0,…,n.
Figure1 illustrates the whole prediction process in mGOASVM for all the three cases: (1) using accession numbers only, (2) using sequences only and (3) using both accession numbers and sequences. Part II does not exist for Case 1, and Part I does not exist for Case 2. Both Part I and Part II exist for Case 3. Score fusion is the fusion of GO scores obtained from accession numbers of homologs in Case 2 or from both true accession numbers and accession numbers of homologs in Case 3.
a<URL> our method, we have more than 300 relevant GO terms for the virus dataset. Even for such a small number of explicit GO terms, many proteins have explicit GO terms spanning several classes.eThe iLoc-Plant[42] web-server is not working properly during testing; so we only reported the performance of the Plant-mPLoc[41] web-server.f<URL>
This work was in part supported by The HK RGC Grant No. PolyU5264/09E and HKPolyU Grant No. G-U877.
WSB designed the system, implemented programs and participated in manuscript preparation. MMW carried out the analysis, reviewed the study and participated in manuscript preparation. KSY supervised the whole project and participated in manuscript preparation. WSB and MMW conceived the idea of this work. All authors read and approved the final manuscript.


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“” he said. “It’s a lot harder than people think, and it’s ag I’ll never forget.” </s> Futsal football differs in many ways from football on a grass pitch. It relies more on quick reactions, passing and technical finesse - and this is typically done at a really high tempo. To get the most out of it, we recommend that you invest in an indoor football. Indoor balls are specifically designed for futsal and moving extremely fast across the ground. You will find our selection of indoor footballs right here, and you'll be ready to dominate in futsal and the indoor courts. </s> Transfer rumour analysis: Thomas Partey to Manchester United
We tracked the rumours to their original source, and assess whether the 25-year-old could be Old Trafford bound.
Daniel Jimenez happened to let slip to passioneinter.com: “The termination clause in Thomas’s contract is 50 million euros, and that’s a cheap figure for a player like him.”
This was picked up by national papers in England, with Partey touted as a United target.
Then 24 hours later, Football Ghana claimed United were ‘set to’ activate the release clause.
“That is because .”
Partey himself played it more cool. He told the BBC: y.”
Do Manchester United want Thomas Partey?
The claims United were set to activate his release clause were first published at the start of the week, and nothing has happened.
He will be featuring for Ghana in the upcoming African Cup of Nations. His agent is making sure Partey is in the shop window by raising his profile.
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Shanray van Wyk is a comedian, actor, and writer. While he says he’s lazy, his output says otherwise. Shanray kept busy during lockdown, and thanks to his nightly Instagram Live shows he is currently the face of Outsurance. Run George, a short film he wrote with Bongani Dube, was also finished during the pandemic and has been picked up by festivals around the world. He’s also back to performing his one-man show, Come I Tell You, after the pandemic brought a successful first run to an end.
In this podcast, we hear what it was like to manage the Melville Comedy Club, how lockdown brought everything to a halt, and what the journey of getting back on stage has been like for him. Shanray shares the particularly brutal criticism he and Bongani got about the first draft of Run George from an older comedian and how it motivated them. We hear about his rugby career that saw him playing provincially after only starting in matric. And we hear how giving that up that career influenced everything going forward. Enjoy.
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22 Ways You Are Amazing I created this post with you in mind. However, in full disclosure, I have an ulterior motive. I’ll reveal the ulterior motive at the end. Here’s what I think of you you’re amazing, believe it: You make everyday a masterpiece. The life you want is already here, embrace it so […
Ared?[…
Beginning Step on How You “Lose Weight” It’s astounding how much we sabotage ourselves with our thoughts. There are so many times we allow our thoughts to go rogue on us. We don’t think about what we are saying. When it comes to losing weight, think about that word, lose or lost. Did you lose weight?Do […
The video is about finding gratitude in life. In the article, I share a story about abuse and how she overcame the struggle. Grateful – Lessons Learned From Abuse The other day I was talking to someone who had experienced some awful things as a child. She was over and […
Learning to Manage Your Emotions is Critical We are continually being bombarded by negativity, just turn on any news and see for yourself. Political turmoil, harm, murder, right, wrong, movies, music, there’s a lot that’s all negative, which causes you to fill up with negativity faster. You already started out the day rough because something […
Stop Hate Know Where You’re Going Hate destroys you more than it does getting revenge. Too many times we don’t manage our emotions and emotions manage us. We are reactive instead of proactive in controlling them. Today’s video and podcast I’m comparing the movie, “Miracle” with life. Miracle is a Disney movie about the 1980 […
Managing Emotions of Pregnancy – How to Keep Going!There are many emotions that you feel when you’re pregnant. Learning how to manage the emotions of pregnancy like postpartum depression, anxiety, overwhelm, fear and frustration is challenging but possible. It’s astounding to me the miracle of children and childbirth. I’ve watched my spectacular wife give […
!Last night we got dumped on from above, we have more snow than I have seen in American Fork in the past 20 years, it’s awesome. We got a dog for Christmas and when I let the dog outside, he took off. He […
Analyze and Balance Life I count my blessings continuously as I’ve found balance in life. Recently, I have been analyzing and designing my life in detail, in more detail than I ever have. I have been going through a program to help me balance life, which is intense, walking me through each aspect of my […
Are You Making the Right Decision?This question about making decisions is a paralyzing question, because you might be afraid of making the wrong decision. If you follow the process I’ve outlined below, you may start making decisions easier and without all of the frustration. My name is Tony Rhoton, and I’m here to help […


Police said six reports have been filed and they are askingon the matter.
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I’m back from Eid holidays, and it’s been a refreshing moment with family and friends. Well, it’s great to be back, especially to trade the US Nonfarm payroll data. On Friday, the greenback is set for its worst week since March ahead of US jobs data that is seen supporting chances of a US interest rate cut. Whereas, the single currency Euro continued to be stronger w.
On the first Friday of every month, the US market releases the nonfarm payroll and unemployment rate, which is often known for shaking the market. Considering the market fluctuations this week, we do need a fundamental to trigger corrections in the market today, and in the coming week, US NFP can be the one. Let’s take a look.
 12:30 GMT. After a substantial growth of 263k jobs, the US economy is expected to add only 180k jobs in May. Whereas, th3.6%. For sure, it’s not something exciting. Thus it may keep the dollar under pressure.
On Wednesday, job creation skidded to a near-halt in May in another sign that the US economic momentum is slowing. Companies added just 27K new positions during the month. It appears that the greenback may strengthen on the news release (180k forecast) as most investors are expecting a figure under 100K.
Average Hourly Earnings m/m – May, the earnings slipped from 0.3% forecast to 0.2%, signaling slacks in the labor market, but economists are optimistic about this months report. The figures are expected to jump by 0.3% from 0.2%.
Statistics Canada will be releasing the capacity utilization rate at 12:30 GMT. The data showbuilders, mines, oil extractors, and utilities. Whedata is expected to be 81.0%, slightly down from 81.7% previously.
At 12:30 GMT, Statistics Canada will be releasing the Canadian unemployment rate. It’s forecast to remain unchanged at 5.7%. Whereas, employment change is something in focus. Statistics Canada reported a surge in Employment Change by 106.5k jobs in May.
All the best for today and stay tuned for more updates!


The Recorder was founded in 1898 and the paper’s motto is “You can’t beat a local”. The paid paper is published bi-weekly and the main circulation areas are Port Pirie, Crystal Brook, Gladstone, Melrose and Port Broughton. Some 15,000 people live in the region, which is geographically positioned beside Spencer Gulf in the mid north of South Australia. The main tourist attractions are the Flinders Rangers and fishing in the gulf. The world’s biggest smelter is located at Port Pirie and farming is the other major industry. Actor Keith Michell and footballer and former Crows captain, Mark Bickley are the area’s most famous locals. The Smelter’s Picnic, held in Spring, is the most popular community event. Source
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portpirierecorder.com.au — =MURRAY BRIDGE: Directors Mari Reu, Trent Baker and Robyn Bates show some razzle dazzle on the opening night of Murray Bridge Players and Singers' production of Chicago. Photo: Peri StrathearnBORDERTOWN: Myah, Airlie, Ella and Braiden Colwill line up to choose their show bags at the 2017 Bordertown Show. Photo: Hamish Ferguson.
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portpirierecorder.com.au — More than 730 residents have rushed to show their interest in joining a $19-a-week solar-power scheme pioneered by Port Pirie Regional Council. The scheme will now move to the next stage of determining contractors to instal the units Council’s corporate and community director James Holyman said his team would analyse applications from Monday.
portpirierecorder.com.au — Tori- Anne Gill is as per any girl who comes from the country, but in between scrubbing sheep troughs and driving the header, she is busy working away on her label, Dark Thorn, preparing for September 2018, when models will debut her label in Paris Fashion Week. The 22-year-old from Mundoora started printing t-shirts in 2013, in a studio out the back of her family farm and has now expanded to an online store where all three of her collections are for sale.
portpirierecorder.com.au — Fire-fighters got up close and personal in Port Pirie with their most-dreaded scenario – the moments before a “flash-over”. In a converted shipping container, five part-time fire-fighters saw how a blaze can evolve into a situation where everything combustible suddenly ignites. “The ‘flash-over’ is the worst-case scenario,” said instructor and station officer Stuart Helmore, of Adelaide.
portpirierecorder.com.au — The Bosco Prize exhibition was opened on Tuesday at St Mark’s college and features 50 pieces of work from visual art and design students. The college has been exhibiting Year 12 visual art work for 15 years, but has recently expanded the exhibition to encompass work from all secondary year levels, allowing younger students to have the confidence to exhibit their pieces. This year the senior prize was awarded to Angel Allan for her couture wire gown and sculpture named ‘Hummingbird’.
Risdon Park men arrested over drugs and firearms
portpirierecorder.com.au — Two men from Risdon Park South were arrested on Wednesday for drug, weapons and firearm offences. Port Pirie patrols were called to an alarm at Airdale Primary School when they located a man riding away from the school. The man was stopped and searched and was allegedly found carrying a taser, quantity of methamphetamine and a small amount of cannabis. The 24-year-old man was arrested for possessing a prohibited substance that was for sale, possessing a taser and also a prescribed firearm.
By Brendan Simpkins
portpirierecorder.com.au — n Wednesday morning which showed that 61 per cent of respondents to the voluntary postal surveMr Hay said he felt “drained” after the long process, but was happy with the result of the survey. “I was in the car park at Woolworths when I heard the news,” he said. “My first reaction was ‘my God’, it was just strange.
portpirierecorder.com.au — The 50kmh speed limit in the “new” Florence Street area will remain unchanged. This is despite a resident calling for a reduction in the limit because the area is congested with cars and pedestrians. The resident said cars reversing from parking spots presented a particular challenge to vehicles that might be travelling at 50kmh. “They could end up in the boot,” he said. The street between Norman Street and Alexander Street was upgraded in a $4 million project by Port Pirie Regional Council.
#LoveIsLove: South Australians vote yes
portpirierecorder.com.au — South Australia has thrown its support behind same-sex marriage, in line with the national survey results revealing a huge 61.6 per cent of people voted yes. Of 951,553 voters in South Australia, 592,528 people, or 62.5 per cent, voted thecomparison, 356,247 people, or 37.5 per cent, voted against. Nearly eight out of 10 eligible Australians in SA, or 79.7 per cent, expressed their view.
Town rallies behind communication partners
portpirierecorder.com.au — A television commercial has sparked interest from people in becoming communication partners in the local court system. Jennifer Jacobs, of the Communication Partner Service with Uniting Communities, travelled to Port Pirie from Adelaide recently to gauge support for the venture. The scheme involves communication partners being assigned to help people with complex communication difficulties to navigate their way through court appearances and legal proceedings.
The Recorder Journalists
Abelson, Justin


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This is a list of all 11 letter words that start with the letter o contained in the litscape.com default word list.
(0.514615 % of all words in this word list.)
oarsmanship obesophobes obesophobia obesophobic obfuscating obfuscation obfuscators obfuscatory obituarists objectbased objectified objectifies objectivate objectively objectivise objectivism objectivist objectivity objectivize objectmaker objurations objurgating objurgation objurgative objurgators objurgatory obligations obligements obliqueness obliquities obliquitous obliterated obliterates obliterator obliviously obnoxiously obsagittate obsceneness obscenities obscurantic obscuration obscurative obscuratory obscurement obscureness obscurities obsecrating obsecration obsecratory obsequences obsequially obsequience obsequities observables observances observantly observation observatory obsessional obsessively obsolescent obsolescing obstetrical obstetrists obstinately obstructers obstructing obstruction obstructive obstructors obtrusively obviousness occasioning occidentals occipitally occultation occupancies occupations occurrences oceanariums oceanfronts oceanologic oceanophile oceanophyte ochlophobes ochlophobia ochlophobic ochophobics ochratoxins octadecamer octagonally octahedrons octapeptide octaploidal octaploidic octarchical octavalence octavalency octavalents octilliards octogesimal octohedrons octoploidal octoploidic octovalence octovalency octovalents octuplicate oculofacial oculomotory oculospinal oddnumbered odonatology odontoblast odontogenic odontograms odontograph odontolites odontoliths odontologic odontomancy odontophobe odontophore odoriferous odoriphores odoriphoric odynophobes odynophobia odynophobic oecological oecologists oecophobics oenanthylic oenological oenologists oenophilist oenophobics oenophobist oesophageal oesophagram offensively offhandedly offhandness officeblock officemates officialdom officialise officialize officiating officiators officiously oikonymical oikophobics olenimorphs olenimorphy oleophobics oligarchies oligarchism oligarchist oligistical oligochaete oligoclases oligocystic oligodactyl oligomycins oligopolies oligopolist oligotrophy olistoliths ololygmancy ombrophobes ombrophobia ombrophobic ombrophytes ombrophytic ombudswoman ominousness ommatophore ommetaphobe omniloquent omniphobics omnipotence omnipresent omniscience omnisexuals omnivalence omnivalency omnivalents omophagists omosternums omphalocele oncogeneses oncogenesis oncological oncologists oncoplastic oncoviruses oneiromancy oneiroscopy onerousness onomastical onomasticon onomatology ontogenesis ontogenetic ontological ontologists onycholysis onychomancy onychophagy oophoridium oophoropexy oophorotomy opacimeters opalescence opalization opeidoscope openhearted openmouthed operability operahouses operational operatising operatizing operculated operculates operettists ophicleides ophiomorphs ophiomorphy ophiophobes ophiophobia ophiophobic ophthalmics ophthalmist opinionated opinionator opisometers opisometric opisthionic opisthokont opportunely opportunism opportunist opportunity oppositions oppressions opprobrious opsomaniacs optimalised optimaliser optimalises optimalized optimalizer optimalizes optionality optokinetic optometrist optophobics orangeberry orangutangs orbicularly orbitostats orbitostyly orchardists orchestrate orchidopexy orchiectomy orderedness orderliness ordinations oreologists organically organisable organizable organologic organonymal organonymic organoscopy organotroph orientalise orientalist orientality orientalize orientating orientation orientative originalism originalist originality originarily originating origination originative originators ornamentals ornamentary ornamenters ornamenting ornamentist ornithology ornithopods oroelogical orogenesies orographers orographies orthoclases orthodeoxia orthodontia orthodontic orthodoxies orthogneiss orthographs orthography orthologous orthonormal orthopaedic orthopedics orthopedist orthopinene orthopnoeas orthopnoeic orthoprisms orthoptists orthoscopes orthoscopic orthostatic orthostyles orthostylic orthotropic orthotypous orthoxazine orthoxylene orthozygous oryctomancy oscillating oscillation oscillators oscillatory oscillogram osculations osmophobics osmophorous osmotically osmotrophic osseomucoid ostectomies ostentation osteoblasts osteoclases osteoclasia osteoclasis osteoclasts osteoclasty osteologist osteopathic osteophages osteophagia osteophores osteophytes osteophytic osteoplaque osteoplasts osteoplasty osteoscopes osteosuture osteotomies ostracisers ostracising ostracizers ostracizing ostrichlike otoabraders otoplasties ototoxicity ouranomancy ouranophobe outachieved outachieves outbabbling outbalanced outbalances outbargains outbartered outbelching outbellowed outbitching outbleating outbleeding outblessing outblooming outbluffing outblushing outblusters outboasting outbragging outbrawling outbreaking outbreathed outbreather outbreathes outbreeding outbuilding outbullying outbursting outbustling outcaviling outcavilled outcharging outcharming outcheating outclassing outclimbing outcoaching outcompeted outcompetes outcounting outcrawling outcroppers outcropping outcrossing outcrowding outdazzling outdebating outdelivers outdesigned outdistance outdoorsman outdoorsmen outdragging outdreaming outdressing outdrinking outduelling outfeasting outfielders outfielding outfighting outfiguring outfiltered outfittings outflanking outflinging outfloating outfrowning outfumbling outgalloped outgambling outgenerals outgleaming outglitters outgrinning outgrossing outguessing outhandling outhumoring outhumoured outhustling outjettings outjockeyed outjourneys outjuggling outjuttings outlaboring outlaboured outlaughing outlaunched outlaunches outlearning outlineless outmaneuver outmarching outmarriage outmarrying outmastered outmatching outmeasured outmeasures outmuscling outnumbered outorganise outorganize outpainting outpatients outperforms outpitching outplanning outpleasing outplodding outplotting outpolitick outpopulate outpouching outpourings outpowering outpractice outpraising outpreached outpreaches outpreening outpressing outproduced outproduces outpromised outpromises outpunching outpursuing outquibbled outquibbles outreachers outreaching outreasoned outrebounds outreckoned outreigning outriggered outriggings outrivaling outrivalled outsavoring outsavoured outscheming outscolding outscooping outscorning outscouring outscreamed outsearched outsearcher outsearches outshooting outshouting outskipping outsleeping outsmarting outsmelling outsourcing outspanning outsparkled outsparkles outsparring outspeakers outspeaking outspeeding outspelling outspending outspinning outspokenly outsporting outsprinted outstanding outstartled outstartles outstations outstealing outsteering outstepping outstrained outstriding outstriking outstripped outstriving outstrutted outstudying outswearing outsweetens outswimming outswindled outswindles outswingers outswinging outthanking outthieving outthinking outthrowing outthrusted outtowering outtraveled outtricking outtrotting outtrumping outwarbling outwardmost outwardness outweighing outwhirling outwiggling outworthing outwrangled outwrangles outwrestled outwrestles outwriggled outwriggles outyielding ovenproofed ovenproofer overabsorbs overabusive overaccents overachieve overacidity overactions overadjusts overadorned overaerated overaerates overaffects overaffirms overagitate overagonize overamplify overanalyse overanalyze overanxiety overanxious overapplied overapplies overarching overarousal overaroused overarouses overasserts overassumed overassumes overassured overbalance overbathing overbearing overbeating overbidders overbidding overbilling overblanket overboiling overbooking overborrows overbracing overbraking overbreathe overbreaths overbulking overburdens overburning overcapable overcareful overcasting overcaution overcensors overcertify overcharged overcharges overchecked overchecker overchilled overchoking overcladded overclaimed overclasped overcleaned overcleaner overcleared overclocked overclothed overclothes overclouded overcloying overcluster overclutter overcoached overcoaches overcoating overcoddled overcoddles overcollect overcolored overcolours overcomable overcommits overcomplex overconcern overconsume overcontrol overcooking overcooling overcorrect overcosting overcounted overcropped overcrowded overcurious overcurrent overcutting overdarkens overdazzled overdazzles overdebated overdebates overdeclare overdefends overdefiant overdeliver overdenture overdepends overdepress overdesigns overdetails overdetects overdevelop overdeviate overdiffuse overdignify overdignity overdilated overdilates overdiluted overdilutes overdirects overdiscuss overdistend overdosages overdotting overdraught overdrawers overdrawing overdredged overdredges overdressed overdresses overdriving overdrugged overdubbing overdusting overeagerly overearners overearnest overearning overediting overeducate overelevate overemoting overemulate overendowed overexcited overexcites overexerted overexhaust overexpands overexpects overexplain overexploit overexposed overexposes overexpress overextends overextract overextreme overfarming overfatigue overfavored overfearful overfeeding overfillers overfilling overfilters overfinance overfishers overfishing overflavors overflavour overflights overflooded overflowing overflushed overflushes overfocused overfocuses overfolding overfraught overfreight overfulfill overfunding overfurnish overfurrows overgarment overgarnish overgearing overgeneral overgilding overglanced overglances overglasses overglazing overgrasped overgrazing overgrinded overgroomed overgrounds overgrowing overgrowths overhandled overhandles overhanging overhardens overharshly overharvest overhaulers overhauling overheaping overhearers overhearing overheating overhelpful overhostile overhunting overhydrate overimitate overimposed overimposes overimpress overincline overindulge overinflate overinforms overinsured overinsurer overinsures overintense overinvests overinvolve overiodized overionized overissuing overjackets overjealous overjudging overjumping overjustify overkeeping overkilling overlabored overlabours overlactate overlanders overlappers overlapping overlayings overleading overleaning overleaping overlearned overleavens overlending overlengths overlighted overlightly overlisting overloading overlockers overlocking overlookers overlooking overmagnify overmanaged overmanages overmarkets overmarking overmasters overmatched overmatches overmeasure overmelting overmilking overmoisten overmolding overneglect overnighted overnighter overnourish overobesely overobesity overoperate overoxidize overpackage overpacking overpainful overpainted overpayment overperform overpitched overpitches overplanned overplayful overplaying overpleased overpleases overplotted overpoliced overpolices overpowered overpraised overpraises overprecise overprepare overpressed overpresses overpricing overprinted overprocess overproduce overpromise overpromote overprotect overprovide overprovoke overpruning overpumping overqualify overreached overreacher overreaches overreacted overreactor overrealist overrecords overrecruit overreduced overreduces overrefined overrefines overrelaxed overrelaxes overreliant overreports overrespond overrestore overretains overrewards overripened overroasted overrulings overrunning oversadness oversalting oversampled oversampler oversamples oversanding overscaling overscorers overscoring overscreens overseasons oversecrete oversedated oversedates overseeding overselling overservice overserving oversetting oversettled oversettler oversettles overshading overshadows overshining overshorten overshowers oversitting overskilled overslavish overslipped oversmoking oversoaking overspanned overspecify overspended overspender overspicing overspilled overspinned overspinner overspreads overstaffed overstained overstaring overstating overstayers overstaying oversteered overstepped overstiffen overstiffly overstifled overstifles overstirred overstocked overstrains overstretch overstrides overstrikes overstudied overstudies overstuffed overstuffer oversudsing oversupport overswaying oversweeten oversweetly overswelled overtakable overtalkers overtalking overteaches overthickly overthought overthrifty overthrower overthrusts overtighten overtightly overtilling overtilting overtipping overtopping overtraders overtrading overtrained overtreated overtricked overtrimmed overtrouble overtrusted overturners overturning overtwisted overutilise overutilize overvaliant overvaluing overviolent overvividly overvoltage overvolting overwalking overwarming overwashing overwatched overwatcher overwatches overwatered overweather overweighed overweights overwetness overwetting overwhelmed overwhelmer overwhipped overwilling overwinding overwinters overworkers overworking overwrapped overwriting overwritten overwrought overzealous ovicapsular ovicapsules oviparities oviparously ovipositing oviposition ovipositors ovoidshaped oxadiazoles oxalacetate oxaldehydes oxalisation oxalization oxaloacetic oxanilamide oxazolidine oxfendazole oxidational oxidatively oxidimetric oxidisables oxidisation oxidisement oxidizables oxidization oxidizement oxprenolols oxyaldehyde oxybenzenes oxybromides oxycyanides oxygenating oxygenation oxygenators oxygenerate oxygenicity oxygenisers oxygenising oxygenizers oxygenizing oxyhaematin oxyhexaster oxyhydrates oxyhydrogen oxylophytes oxylophytic oxymandelic oxymuriates oxymuriatic oxynaphthol oxynitrates oxyphthalic oxysulfides oxysulphate oxysulphide oxysulphids oxyterpenes oxytoluenes oxyuricides oysterhouse oystershell oysterwoman oysterwomen ozonisation ozonization ozonometers ozonometric ozonoscopes ozonoscopic ozonosphere
11 letter o words   at www.litscape.com
11 letter o words at www.litscape.com


In the six years we've known Robby and Cari Rowe, Co-Owners of Phoenix Campers, we have learned to expect the unexpected. In the recent past, Robby has rocked the RV marketplace with Chevy Avalanche Campers, Ford Raptor Campers, and Jeep Campers of every description. He's fearless about creating totally custom campers for almost any vehicle his customers bring him, and equally fearless about making their wildest camper dreams come true. Have a unique idea for a camper?
This brings us to the subject of today's story, the Bronco Bronco. In a nutshell, the Bronco Bronco is a Denver Bronco themed camper built on, what else?A Ford Bronco!All at once the Bronco Bronco seems like the craziest and the most obvious camper we've ever seen. Crazy because, well, just look at it. This may be the most over the top camper ever made. And obvious because, well, someone had to build a Bronco Bronco, right?See what we mean be expecting the unexpected?
TCM: Is the Bronco Bronco specifically for tailgating at Bronco football games, or will it also be used for actual camping?
TCM: How did you design the exterior look of the Bronco Bronco?
Robby: When the 40-inch HDTV showed up at the office one day, Cari asked, "Where is that going on the camper?
TCM: What's on the back hatch?
TCM: The upholstery and stitching in the truck seats and camper dinette is incredible. How did you do that?
TCM: What's the story behind the unusual shape of the dinette seating?
TCM: In the photography, it appears that the catalytic heater under the bathroom entry is the only source of heat. Is that enough to heat the whole unit?
TCM: If someone wants a camper like the Bronco Bronco, can they get one from Phoenix Campers?
TCM: What was the reaction to the Bronco Bronco at the Denver ISE Show?
TCM: What's next for the Bronco Bronco?Are there any events planned?


 Heit unfriendly.
Wallace has two children with wife Brody: Kelvin aged 3 and Kristy aged 5.
Wallace's best friend is a burglar called Kirsty SheppardBlake Byrne and Robert Reed. They enjoy stealing candy from babies together....


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“Omniwisdom” is a term my young friend Natán made up. He was sharing how God’s ways are higher than ours and made up this word to describe the concept. I loved this new word and decided to look into God’s “omniwisdom.”
The prefix omni is used for three of God’s attributes:
- Omnipotent—God is all-powerful. He can do anything He wants to do.
So, let’s take a look at God’s wisdom.
God is the Source of wisdom.

Wisdom is one of the gifts of the Holy Spirit.Wisdom is one of the gifts of the Holy Spirit.
1 Corinthians 12:8a).
God gives wisdom to people, and it comes from knowing God’s Word.
5).
).
- (Proverbs 1:2-3).

In heaven, wisdom will be one of the offerings given back to Jesus:
God does indeed have “omniwisdom.” He’s the source of wisdom, and He wants to share it with us. He’s a never-ending Fountain, and if we need wisdom, we have only to ask for it. The Bible says He gives wisdom to all men liberally. Do you want to be happy? Get wisdom! Do you want to have a good testimony? Walk in wisdom. Do you want to know God’s will? Get into His Word. Do you need help in your family? God’s wisdom will guide you.
Thank you, Carol. I enjoy doing Bible studies. God bless you!


In today’s world, men can be both villains and victims of the patriarchy — although we refuse to admit that sometimes. Personally, feminism isn’t only about fighting for women’s rights. It’s about making all areas of life equal for all genders. And men’s issues ultimately count toward that, too.
I have a confession to make. nd.


Must be a video submission.
One video per submission.
Accepting group poems.
August 31st, 2022.


ation is integrally formed；Place the mode of contact pin and contact pin insulating base compared with convention connectors contact pin apparatus respectively, more just Victory, effectively；
In sumeing used for replacing convention connectors contact pin apparatus on adapter, realize connecting The automatic running of device contact pin work, has high degree of automation, stable, percent defective is low, and homogeneity of product is high, and efficiency is good etc. Advantage.
Fig. 1 is  one；
Fig. 3 is the integrally-builtone；
 technical scheme inly illustrated.
As shown in Figure 4 and Figure 5, described contact pin fixed mechanism 5 includes contact pin 51, contact pin insulating base 52 and contact pin fixed block 53, the upper end of contact pin fixed block 53 is plugged with several contact pins 51, and the top 511 of this contact pin 51 both passes through contact pin insulating base 52 Upper end and contact pin fixed block 53 is placed in inside contact pin insulating base 52, using contact pin fixed block, makes contact pin and contact pin insulating base reliable Be fixed at one, facilitate later stage contact pin work carrying out.
As shown in figure 3, the described dorsal part pushing fixed seat 25 is provided with guiding mechanism 6, this guiding mechanism 6 includes line slideway 61 and slide block 62, line slideway 61 is anchored in frame 1, is arranged with slide block 62 thereon, and this slide block 62 is with to push fixed seat 25 tight It is solidly connected, limit the degree of freedom during press mechanism pushes.
As shown in figure 3, the described side reclaiming spring 4 is fixed on and is pushed in fixed seat 25, opposite side is fixed on fixed block On 23, using the characteristic reset press mechanism of spring elongation contraction.
Work process is as follows：
Initial contact pin 54, in one end insertion contact pin fixed block 53, the other end 541 insert contact pin fracture block 7 flat recess in Repeatedly after bending 3-4 time, it is inserted in contact pin fixed seat 53 using contact pin insulating base 52 along the top 511 of contact pin 51, forms contact pin solid Determine mechanism 5；Contact pin mechanism 5 grafting enters in the groove 341 of push block 34, and subsequent delivery device 3 works, and push cylinder 31 pushes away Send block 34 level to push to the lower section of press mechanism 2, be blocked bar and stop stopping；Press mechanism 2 is started working, lower air cylinder 21 Knee-piece 22 is driven to rotate around pin joint A, the knee-pie


Twitter tests 'original tweeter' tag to help users see who started a thread
g/092E2E572A994B1C9F40CE45332D0817/afp-20181014-abe-military.jpg
JAPAN'S MILITARY. Japanese Prime Minister Shinzo Abe (L) reviews Self-Defense Force soldiers dse Force', on October 14, 2018. File photo by Kazuhiro Nogi/AFP
The new plans come after pledges from Japan to buy more US military equipment,  Washington's huge trade deficit with Tokyo.
Abe
"That's not going to happen." – Rappler.com
Filed under:China•Japan defense•Japan military•Shinzo Abe•World War II•defense•Japan


ancy. The massage provides a very safe, loving and caring experience to both mother and baby during this precious time of your life, check out our offer for a 4-handed pregnancy Lom
Bullying powers have now turned to a “soft invasion” as part of a second phase of hostilities against the Iran since they failed to defeat the Islamic Republic on the battleground, the Leader said.
In this stage, the global hegemony seeks to exert influence on the country’s “decision-making centers,” and to make changes in people’s beliefs as well as the stances and policies of Iranian officials, the Leader stressed.
Ayatollah Jannati was chosen for the post for two years after securing re-election for the assembly in a February vote. </s> what’s this? | there's no place like gnome.
It’s an egg topper and when I saw it at Crate and Barrel yesterday, I knew I needed it. [Needed being the word naturally in question.] What do I need it for? </s> Consumers spent $6.22  data, up 23.6% compared with $5.03 billion last year. Meanwhile, retailers offered Black Friday-level deals on Thanksgiving.
Black Friday online sales numbers are in—and they grew more than 20% compared to 2017. Meanwhile, Thanksgiving posted an even higher growth rate at nearly 30%, and early Saturday online shopping numbers look strong, too.
Black Friday was also the first day to generate according to Adobe. That followed a $1 billion dollar mobile commerce day on Thanksgiving.
And consumers didn’t seem to tire of web shopping after Friday. For example, as of 10 a.m. Eastern Standard Time Saturday, consumers had spent $400 million online, an increase of 24.3% from a year earlier. Adobe estimates Saturday will rake in $3 billion in web sales but won’t release its official Saturday e-commerce sales figures until 10:30 Eastern Standard Time on Cyber Monday.
As of Black Friday, the steepest discounts were on computers (discounted 17.8% on average), followed by TVs (down 18%) and toys (down 27.1%), Adobe says. Adobe predicts the best deals on Cyber Monday will be on toys.
Meanwhile, e-commerce platform provider and marketing vendor Salesforce.com predicts Black Friday—not Cyber Monday—will be the biggest online holiday shopping day. “The day after Thanksgiving saw a conversion spike of 37%, suggesting it will remain the top digital shopping day of this holiday season,” Salesforce says. , Salesforce says, up from 61% on the same day last year, and accounted for 49% of orders. This share is slightly lower than the 54% mobile order share on Thanksgiving Day.
Salesforce’s data is based on combined the garnered from e-retailers that use Commerce Cloud, Salesforce’s e-commerce platform. It also is based on consumer engagements and public social media conversations tracked through Salesforce’s marketing platform, Marketing Cloud, a, the vendor’s customer service platform. Salesforce’s findings also rely on ’s “Shopper-First Retailing” report with Publicis.Sapient.
Shopify merchants generated more than $870,000 in sales per minute during Black Friday and generated more than $37 million sales per hour at the peak of Black Friday. Additionally, 63% of orders at Shopify merchants stemmed from either phones or tablets, while desktop sales accounted for 37%. 2018 Black Friday gross merchandise value for Shopify merchants surpassed the total Shopify GMV of Black Friday 2017 by 2:30 p.m. Eastern Standard Time. The average order value during Black Friday was $86.37,


 No one likes working weekends, right? There are certain industries that require work 24 X 7 and you just can't help it. We crib, we complain but since there's no way out, here are 11 things that you will relate to if you work on weekends.
You have forgotten what a weekend means anymore. For you, week off is a more appropriate term. Even though it sucks, you have made your peace with it.
Since nobody is free on the days you are free, it's tough to plan a day out with your friends and family. You spend so many days planning and plotting, but more often than not it turns out to be a flop show.
Probably the only good thing about working on a weekend is empty metro and roads. On weekdays it takes you hours to reach your workplace, considering you have to wait for a metro that isn't oozing humans; or you're stuck in endless traffic jam. Travelling is actually easier on weekends.
For you, it's more like Fri-whaaa... roll down, right?
Since your week offs are during the week, the malls are empty and you don't have to stand in queues to try that outfit you picked. It's less crowded and so much better.
7. "Have a happy weekend!" - NO.
Whenever a shopkeeper smiles and tells you to have a happy weekend, you just groan, coz' you ain't getting any weekend bro.
8. Whine. Whine. Whine.
Partying with your work friends is the only option left for you. They are the only ones who are free when you are free. To be honest, it's easy and fun too.
Every time you step into your office on a weekend, you just don't want to work and by the afternoon, this feeling is too real for you to work. So you laze around and try to get off work as soon as you can.
...and that's pretty awesome. While the world complains about having Monday blues, you are pretty chill with your life.


The Malta Jazz and will include free events for all in the city centre. The festivals.